Human histone cluster 1, H4a ELISA Kit | HIST1H4A elisa kit

Human Histone H4, HIST1H4A ELISA Kit

Cat. Num. AAA937969

• Gene Names: HIST2H4B; H4/o
• Reactivity: Human
• Synonyms: histone cluster 1; H4a; Human Histone H4; HIST1H4A ELISA Kit; Human Histone H4 (HIST1H4A) ELISA kit; H4/a; H4FA; H4 histone family; member A; histone 1; HIST1H4A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human HIST1H4A. No significant cross-reactivity or interference between human HIST1H4A and analogues was observed.
• Sequence Length: 103

• Samples: Serum, plasma, tissue homogenates and Cell lysates
• Assay Type: Sandwich
• Detection Range: 37. 5 pg/ml -2400 pg/ml.
• Sensitivity: The minimum detectable dose of human HIST1H4A is typically less than 9.3 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HIST1H4A elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HIST1H4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIST1H4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIST1H4A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIST1H4A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 77539758
• NCBI GeneID: 554313
• NCBI Accession #: NP_001029249.1
• NCBI GenBank Nucleotide #: NM_001034077.4
• UniProt Accession #: P62805; P02304; P02305; Q6DRA9; Q6FGB8; Q6NWP7; A2VCL0
• Molecular Weight: 11,367 Da
• NCBI Official Full Name: histone H4
• NCBI Official Synonym Full Names: histone cluster 2, H4b
• NCBI Official Symbol: HIST2H4B
• NCBI Official Synonym Symbols: H4/o
• NCBI Protein Information: histone H4; histone 2, H4b
• UniProt Protein Name: Histone H4
• UniProt Gene Name: HIST1H4A
• UniProt Synonym Gene Names: H4/A; H4FA
• UniProt Entry Name: H4_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq, Jul 2008]

Uniprot Description

H4: histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The gene encoding H4 is intronless.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: nucleoplasm; nuclear chromosome; protein complex; membrane; extracellular region; nucleosome; nucleus

Molecular Function: protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: chromatin silencing at rDNA; establishment and/or maintenance of chromatin architecture; DNA replication-dependent nucleosome assembly; regulation of gene expression, epigenetic; negative regulation of megakaryocyte differentiation; nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; DNA replication-independent nucleosome assembly; negative regulation of gene expression, epigenetic; protein heterotetramerization; gene expression; mitotic cell cycle; DNA methylation on cytosine; telomere maintenance