SNRPN sirna

SNRPN siRNA (Mouse)

Cat. Num. AAA8240920

• Gene Names: Snrpn; SMN; Peg4; Pwcr1; HCERN3; 2410045I01Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SNRPN; SNRPN siRNA (Mouse); SMN; Small nuclear ribonucleoprotein-associated protein N; snRNP-N; Sm protein D; Sm-D; Sm protein N; Sm-N; SmN; Tissue-specific-splicing protein; SNRPN sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SNRPN siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 240

• Applicable Applications for SNRPN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SNRPN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SNRPN sirna

siRNA to inhibit SNRPN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 133725810
• NCBI GeneID: 20646
• NCBI Accession #: NP_001076430.1
• NCBI GenBank Nucleotide #: NM_001082961.1
• UniProt Accession #: P63163; P14648; P17135
• Molecular Weight: 24,614 Da
• NCBI Official Full Name: small nuclear ribonucleoprotein-associated protein N
• NCBI Official Synonym Full Names: small nuclear ribonucleoprotein N
• NCBI Official Symbol: Snrpn
• NCBI Official Synonym Symbols: SMN; Peg4; Pwcr1; HCERN3; 2410045I01Rik
• NCBI Protein Information: small nuclear ribonucleoprotein-associated protein N
• UniProt Protein Name: Small nuclear ribonucleoprotein-associated protein N
• Protein Family: SNRPN upstream reading frame protein
• UniProt Gene Name: Snrpn
• UniProt Synonym Gene Names: Smn; snRNP-N; Sm-D; Sm-N; SmN
• UniProt Entry Name: RSMN_MOUSE

NCBI Description

The protein encoded by this gene is one polypeptide of a small nuclear ribonucleoprotein complex, and it plays a role in pre-mRNA processing. Although individual snRNPs are believed to recognize specific nucleic acid sequences through RNA-RNA base pairing, the specific role of this family member is unknown. This protein arises from a bicistronic transcript that also encodes a protein identified as the Snrpn upstream reading frame (Snurf). Multiple transcription initiation sites have been identified and extensive alternative splicing occurs in the 5' untranslated region. Additional splice variants have been described but sequences for the complete transcripts have not been determined. The 5' UTR of this gene has been identified as an imprinting center. Alternative splicing or deletion caused by a translocation event in this paternally-expressed region in human and mouse is responsible for Angelman syndrome or Prader-Willi syndrome due to parental imprint switch failure. [provided by RefSeq, Jul 2008]

Uniprot Description

snRNP N: May be involved in tissue-specific alternative RNA processing events. Belongs to the snRNP SmB/SmN family.

Protein type: RNA-binding

Cellular Component: snRNP U2; snRNP U1; nucleus