Hephaestin Recombinant Protein | HEPH recombinant protein

Recombinant Hephaestin (HEPH)

Cat. Num. AAA2029083

• Gene Names: HEPH; CPL
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Hephaestin; Recombinant Hephaestin (HEPH); HEPH recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; ATRVYYL GIRDVQWNYA PKGRNVITNQPLDSDIVASS FLKSDKNRIG GTYKKTIYKE YKDDSYTDEV AQPAWLGFLGPVLQAEVGDV ILIHLKNFAT RPYTIHPHGV FYEKDSEGSL YPDGSSGPLKADDSVPPGGS HIYNWTIPEG HAPTDADPAC LTWIYHSHVD APRDIATGLIGPLITCKRGA LDGNSPPQRQ DVDHDFFLLF SVVDENLSWH LNENIATYCSDPASVDKEDE TFQESNRMHA INGFVFGNLP ELNMCAQKRV AWHLFGMGNEIDVHTAFFHG QMLTTRGHHT DVANIFPATF VTAEMVPWEP GTWLISCQVNSHFRDGMQAL YKVKSC
• Sequence Length: 1158

• Applicable Applications for HEPH recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 42.1kDa
• Accurate Molecular Mass (KD): 42kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Ala24~Cys366
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 12830539
• NCBI GeneID: 9843
• Molecular Weight: 100,624 Da
• NCBI Official Full Name: hephaestin
• NCBI Official Synonym Full Names: hephaestin
• NCBI Official Symbol: HEPH
• NCBI Official Synonym Symbols: CPL
• NCBI Protein Information: hephaestin
• UniProt Protein Name: Hephaestin
• Protein Family: Hephaestin
• UniProt Gene Name: HEPH
• UniProt Synonym Gene Names: KIAA0698
• UniProt Entry Name: HEPH_HUMAN

NCBI Description

This gene encodes a member of the multicopper oxidase protein family. The encoded protein is involved in the transport of dietary iron from epithelial cells of the intestinal lumen into the circulatory system, and may be involved in copper transport and homeostasis. In mouse, defects in this gene can lead to severe microcytic anemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

HEPH: May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1. Belongs to the multicopper oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 1.-.-.-; Oxidoreductase

Chromosomal Location of Human Ortholog: Xq11-q12

Cellular Component: perinuclear region of cytoplasm; basolateral plasma membrane; integral to membrane; plasma membrane; intracellular

Molecular Function: ferroxidase activity; copper ion binding; ferrous iron binding

Biological Process: cellular iron ion homeostasis; copper ion transport; iron ion transport; transmembrane transport