Rabbit anti-Human GNL3L Polyclonal Antibody | anti-GNL3L antibody

GNL3L, NT (GNL3L, Guanine nucleotide-binding protein-like 3-like protein) (Azide free) (HRP)

Cat. Num. AAA6303088

• Gene Names: GNL3L; RP11-353K22.1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GNL3L; Polyclonal Antibody; NT (GNL3L; Guanine nucleotide-binding protein-like 3-like protein) (Azide free) (HRP); Guanine nucleotide-binding protein-like 3-like protein; anti-GNL3L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GNL3L antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GNL3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-130 amino acids from the N-terminal region of human GNL3L.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GNL3L Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the GNL3L antibody detected the GNL3L protein (arrow).)

Flow Cytometry (FC/FACS)

(GNL3L Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GNL3L antibody

The protein encoded by this gene appears to be a nucleolar GTPase that is essential for ribosomal pre-rRNA processing and cell proliferation. Two transcript variants encoding the same protein have been found for this gene.

Product Categories/Family for anti-GNL3L antibody

Antibodies; Binding Proteins

References

Rao, M.R., et al. J. Mol. Biol. 364(4):637-654(2006). Du, X., et al. Mol. Biol. Cell 17(1):460-474(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 296317324
• NCBI GeneID: 54552
• NCBI Accession #: NP_001171748.1; NP_061940.1
• NCBI GenBank Nucleotide #: NM_001184819.1
• UniProt Accession #: Q9NVN8
• Molecular Weight: 65,573 Da
• NCBI Official Full Name: guanine nucleotide-binding protein-like 3-like protein
• NCBI Official Synonym Full Names: guanine nucleotide binding protein-like 3 (nucleolar)-like
• NCBI Official Symbol: GNL3L
• NCBI Official Synonym Symbols: RP11-353K22.1
• NCBI Protein Information: guanine nucleotide-binding protein-like 3-like protein; novel GTPase
• UniProt Protein Name: Guanine nucleotide-binding protein-like 3-like protein
• Protein Family: Guanine nucleotide-binding protein-like 3-like protein
• UniProt Gene Name: GNL3L
• UniProt Entry Name: GNL3L_HUMAN

NCBI Description

The protein encoded by this gene appears to be a nucleolar GTPase that is essential for ribosomal pre-rRNA processing and cell proliferation. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010]

Uniprot Description

GNL3L: Stabilizes TERF1 telomeric association by preventing TERF1 recruitment by PML. Stabilizes TERF1 protein by preventing its ubiquitination and hence proteasomal degradation. Does so by interfering with TERF1-binding to FBXO4 E3 ubiquitin-protein ligase. Required for cell proliferation. By stabilizing TRF1 protein during mitosis, promotes metaphase-to-anaphase transition. Stabilizes MDM2 protein by preventing its ubiquitination, and hence proteasomal degradation. By acting on MDM2, may affect TP53 activity. Required for normal processing of ribosomal pre-rRNA. Binds GTP. Belongs to the MMR1/HSR1 GTP-binding protein family.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: Xp11.22

Cellular Component: nucleoplasm; membrane; mitochondrion; cytoplasm; nucleolus; nucleus

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: metabolic process; ribosome biogenesis and assembly