SHC1 sirna

SHC1 siRNA (Mouse)

Cat. Num. AAA826967

• Gene Names: Shc1; Shc; p66; ShcA; p66shc
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SHC1; SHC1 siRNA (Mouse); SHC; SHCA; SHC-transforming protein 1; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1; SH2 domain protein C1; SHC1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SHC1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 579

• Applicable Applications for SHC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SHC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SHC1 sirna

siRNA to inhibit SHC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 164664522
• NCBI GeneID: 20416
• NCBI Accession #: NP_001106802.1
• NCBI GenBank Nucleotide #: NM_001113331.2
• UniProt Accession #: P98083; Q3U2Q7; Q8BFY3; Q8K4C6; Q8K4C7
• Molecular Weight: 46,467 Da
• NCBI Official Full Name: SHC-transforming protein 1 isoform a
• NCBI Official Synonym Full Names: src homology 2 domain-containing transforming protein C1
• NCBI Official Symbol: Shc1
• NCBI Official Synonym Symbols: Shc; p66; ShcA; p66shc
• NCBI Protein Information: SHC-transforming protein 1
• UniProt Protein Name: SHC-transforming protein 1
• Protein Family: SHC-transforming protein
• UniProt Gene Name: Shc1
• UniProt Synonym Gene Names: Shc; ShcA; SH2 domain protein C1
• UniProt Entry Name: SHC1_MOUSE

Uniprot Description

SHC1: an adaptor protein containing a SH2 domain and a PID domain within a PH domain-like fold. Couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Six human isoforms are produced by alternative promoter usage and alternative splicing. Isoforms p66, p52 and p46 (P29353-1, -2, and -3), produced by alternative initiation, variously regulate growth factor signaling, oncogenesis, intracellular oxidant levels, and apoptosis. Isoforms p46 and p52, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex, thus initiating the cytoplasmic proliferative Ras signaling cascade in various non-neuronal systems. Isoform p66 does not mediate Ras activation, but associates with mitochondria where it controls intracellular redox status, mitochondrial permeability, life span, and stress-induced apoptosis. p66 acts as a downstream target of the tumor suppressor p53 and is required for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. P66 deletion in mice decreases the incidence of aging-associated diseases, such as atherosclerosis, and significantly prolongs life span. Participates in signaling downstream of TIE2, the tyrosine kinase receptor for angiopoietin, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis. Interacts with tyrosine-phosphorylated CD3T, DDR2, LRP1, IRS4, SHP, FLT4, PDGFRB, TIE2, TrkA, -B and -C. Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR in vitro via the PID domain. p66Shc is known to be activated by the mutant SOD1 associated with familial forms of amyotrophic lateral sclerosis (ALS), causing a decrease in the activity of Rac1 through a redox-sensitive regulation. In case of oxidative conditions, phosphorylation at S36 of isoform p66Shc, leads to mitochondrial accumulation p66 plays a role in mediating mitophagy and determining neuronal cell fate following acute oxygen glucose deprivation. Isoform p46 is localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization.

Protein type: Apoptosis; Adaptor/scaffold; Motility/polarity/chemotaxis; Mitochondrial

Cellular Component: mitochondrion; cytoplasm; plasma membrane; endosome membrane; nucleus

Molecular Function: insulin-like growth factor receptor binding; protein binding; ephrin receptor binding; protein phosphatase 2A binding; phosphotyrosine binding; neurotrophin TRKA receptor binding; protein complex binding; phosphoprotein binding; receptor tyrosine kinase binding; protein kinase binding; insulin receptor binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; activation of MAPK activity; positive regulation of smooth muscle cell proliferation; heart development; MAPKKK cascade; signal transduction; regulation of cell proliferation; neuron differentiation; cell-cell adhesion; regulation of growth; actin cytoskeleton reorganization; insulin receptor signaling pathway; positive regulation of vasoconstriction; angiogenesis; actin cytoskeleton organization and biogenesis; neurite development; positive regulation of DNA replication; aging