Rabbit anti-Mouse Shc1 Polyclonal Antibody | anti-Shc1 antibody

Shc1, ID (Shc1, Shc, ShcA, SHC-transforming protein 1, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1) (Azide free) (HRP)

Cat. Num. AAA6347355

• Gene Names: Shc1; Shc; p66; ShcA; p66shc
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Shc1; Polyclonal Antibody; ID (Shc1; Shc; ShcA; SHC-transforming protein 1; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1) (Azide free) (HRP); Src homology 2 domain-containing-transforming protein C1; anti-Shc1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Shc1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Shc1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-405 amino acids from the Central region of mouse Shc1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Shc1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Mouse Shc1 antibody detected the Mouse Shc1 protein (arrow).)

Related Product Information for anti-Shc1 antibody

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p47Shc and isoform p52Shc, once phosphorylated, couple activated receptor kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p47Shc and isoform p52 may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span.

Product Categories/Family for anti-Shc1 antibody

Antibodies; Signal Transduction

References

Ursini-Siegel, J., et al. Cancer Res. 70(20):7776-7787(2010). Ma, Z., et al. Oncogene 29(41):5559-5567(2010). Fadini, G.P., et al. Diabetes 59(9):2306-2314(2010). Ranieri, S.C., et al. Proc. Natl. Acad. Sci. U.S.A. 107(30):13420-13425(2010). Gines, S., et al. J. Biol. Chem. 285(28):21537-21548(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 164664522
• NCBI GeneID: 20416
• NCBI Accession #: NP_001106802.1
• NCBI GenBank Nucleotide #: NM_001113331.2
• UniProt Accession #: P98083; Q3U2Q7; Q8BFY3; Q8K4C6; Q8K4C7
• Molecular Weight: 46,467 Da
• NCBI Official Full Name: SHC-transforming protein 1 isoform a
• NCBI Official Synonym Full Names: src homology 2 domain-containing transforming protein C1
• NCBI Official Symbol: Shc1
• NCBI Official Synonym Symbols: Shc; p66; ShcA; p66shc
• NCBI Protein Information: SHC-transforming protein 1
• UniProt Protein Name: SHC-transforming protein 1
• Protein Family: SHC-transforming protein
• UniProt Gene Name: Shc1
• UniProt Synonym Gene Names: Shc; ShcA; SH2 domain protein C1
• UniProt Entry Name: SHC1_MOUSE

Uniprot Description

SHC1: an adaptor protein containing a SH2 domain and a PID domain within a PH domain-like fold. Couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Six human isoforms are produced by alternative promoter usage and alternative splicing. Isoforms p66, p52 and p46 (P29353-1, -2, and -3), produced by alternative initiation, variously regulate growth factor signaling, oncogenesis, intracellular oxidant levels, and apoptosis. Isoforms p46 and p52, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex, thus initiating the cytoplasmic proliferative Ras signaling cascade in various non-neuronal systems. Isoform p66 does not mediate Ras activation, but associates with mitochondria where it controls intracellular redox status, mitochondrial permeability, life span, and stress-induced apoptosis. p66 acts as a downstream target of the tumor suppressor p53 and is required for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. P66 deletion in mice decreases the incidence of aging-associated diseases, such as atherosclerosis, and significantly prolongs life span. Participates in signaling downstream of TIE2, the tyrosine kinase receptor for angiopoietin, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis. Interacts with tyrosine-phosphorylated CD3T, DDR2, LRP1, IRS4, SHP, FLT4, PDGFRB, TIE2, TrkA, -B and -C. Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR in vitro via the PID domain. p66Shc is known to be activated by the mutant SOD1 associated with familial forms of amyotrophic lateral sclerosis (ALS), causing a decrease in the activity of Rac1 through a redox-sensitive regulation. In case of oxidative conditions, phosphorylation at S36 of isoform p66Shc, leads to mitochondrial accumulation p66 plays a role in mediating mitophagy and determining neuronal cell fate following acute oxygen glucose deprivation. Isoform p46 is localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization.

Protein type: Apoptosis; Adaptor/scaffold; Motility/polarity/chemotaxis; Mitochondrial

Cellular Component: mitochondrion; cytoplasm; plasma membrane; endosome membrane; nucleus

Molecular Function: insulin-like growth factor receptor binding; protein binding; ephrin receptor binding; protein phosphatase 2A binding; phosphotyrosine binding; neurotrophin TRKA receptor binding; protein complex binding; phosphoprotein binding; receptor tyrosine kinase binding; protein kinase binding; insulin receptor binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; activation of MAPK activity; positive regulation of smooth muscle cell proliferation; heart development; MAPKKK cascade; signal transduction; regulation of cell proliferation; neuron differentiation; cell-cell adhesion; regulation of growth; actin cytoskeleton reorganization; insulin receptor signaling pathway; positive regulation of vasoconstriction; angiogenesis; actin cytoskeleton organization and biogenesis; neurite development; positive regulation of DNA replication; aging