Human Metabotropic glutamate receptor 7 (GRM7) ELISA Kit | GRM7 elisa kit

Human Metabotropic glutamate receptor 7 (GRM7) ELISA Kit

Cat. Num. AAA7204164

• Gene Names: GRM7; GLUR7; MGLU7; GPRC1G; MGLUR7; PPP1R87
• Reactivity: Human
• Synonyms: Metabotropic glutamate receptor 7 (GRM7); Human Metabotropic glutamate receptor 7 (GRM7) ELISA Kit; GRM7 elisa kit

• Reactivity: Human

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRM7 elisa kit

Intended Uses: This GRM1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GRM1. This ELISA kit for research use only!

Principle of the Assay: GRM1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GRM1 antibody and an GRM1-HRP conjugate. The assay sample and buffer are incubated together with GRM1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GRM1 concentration since GRM1 from samples and GRM1-HRP conjugate compete for the anti-GRM1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GRM1 from the sample, fewer sites are left to bind GRM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRM1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GRM7 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 32528268
• NCBI GeneID: 2917
• NCBI Accession #: NP_870989.1
• NCBI GenBank Nucleotide #: NM_181874.2
• Molecular Weight: 101,302 Da
• NCBI Official Full Name: metabotropic glutamate receptor 7 isoform b
• NCBI Official Synonym Full Names: glutamate receptor, metabotropic 7
• NCBI Official Symbol: GRM7
• NCBI Official Synonym Symbols: GLUR7; MGLU7; GPRC1G; MGLUR7; PPP1R87
• NCBI Protein Information: metabotropic glutamate receptor 7; protein phosphatase 1, regulatory subunit 87
• UniProt Protein Name: Metabotropic glutamate receptor 7
• Protein Family: Metabotropic glutamate receptor
• UniProt Gene Name: GRM7
• UniProt Synonym Gene Names: GPRC1G; MGLUR7; mGluR7
• UniProt Entry Name: GRM7_HUMAN

NCBI Description

L-glutamate is the major excitatory neurotransmitter in the central nervous system, and it activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors that have been divided into three groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5, and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3, while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

mGluR7: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. Belongs to the G-protein coupled receptor 3 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; GPCR, family 3; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 3p26.1-p25.1

Cellular Component: asymmetric synapse; presynaptic membrane; postsynaptic membrane; integral to plasma membrane; axon; dendrite; plasma membrane; presynaptic active zone; integral to membrane; cell cortex; dendritic shaft; receptor complex

Molecular Function: voltage-gated calcium channel activity; group III metabotropic glutamate receptor activity; glutamate binding; calcium channel regulator activity; calcium ion binding; glutamate receptor activity; PDZ domain binding

Biological Process: synaptic transmission; negative regulation of cAMP biosynthetic process; sensory perception of sound; regulation of synaptic transmission, glutamatergic; behavioral fear response; negative regulation of glutamate secretion; short-term memory; conditioned taste aversion; adult behavior; negative regulation of adenylate cyclase activity; sensory perception of smell; regulation of cyclase activity; transmission of nerve impulse; metabotropic glutamate receptor, adenylate cyclase inhibiting pathway