S100A1 sirna

S100A1 siRNA (Mouse)

Cat. Num. AAA8235980

• Gene Names: S100a1; S100; S100a; AI266795
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: S100A1; S100A1 siRNA (Mouse); Protein S100-A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 calcium-binding protein A1; S100A1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: S100A1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 94

• Applicable Applications for S100A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse S100A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for S100A1 sirna

siRNA to inhibit S100A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6755386
• NCBI GeneID: 20193
• NCBI Accession #: NP_035439.1
• NCBI GenBank Nucleotide #: NM_011309.3
• UniProt Accession #: P56565; O88949
• Molecular Weight: 10,505 Da
• NCBI Official Full Name: protein S100-A1
• NCBI Official Synonym Full Names: S100 calcium binding protein A1
• NCBI Official Symbol: S100a1
• NCBI Official Synonym Symbols: S100; S100a; AI266795
• NCBI Protein Information: protein S100-A1
• UniProt Protein Name: Protein S100-A1
• Protein Family: Protein
• UniProt Gene Name: S100a1
• UniProt Entry Name: S10A1_MOUSE

Uniprot Description

S100A1: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. Interacts with AGER and CAPZA1. Interacts with FKBP4. Interacts with RYR1 and RYR2. Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. Belongs to the S-100 family.

Protein type: Endoplasmic reticulum; Calcium-binding

Cellular Component: I band; neuron projection; sarcoplasmic reticulum; cytoplasm; M band; nucleus; Z disc; A band

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; metal ion binding; calcium ion binding; calcium-dependent protein binding; ATPase binding

Biological Process: negative regulation of transcription from RNA polymerase II promoter; regulation of heart contraction