S100A1 recombinant protein

S100A1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8118836

• Gene Names: S100a1; S100; S100a; AI266795
• Purity: >95 % as determined by SDS-PAGE
• Synonyms: S100A1; S100A1 Protein; Mouse; Recombinant (His Tag); Mouse S100A1 Protein (His Tag); S100 calcium binding protein A1; AI266795 Protein; S100 Protein; S100a Protein; S100A1 recombinant protein

• Host: E Coli
• Purity/Purification: >95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 109aa
• Sequence: Gly2-Ser94

• Species: Mouse
• Tag: N-His
• Predicted N Terminal: Met
• Protein Construction: |A DNA sequence encoding the mouse S100A1 (P56565) (Gly 2-Ser 94) was expressed, with a polyhistide tag at the N-terminus
• Activity: Measured by its ability to bind human AGER in a functional ELISA.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Related Product Information for S100A1 recombinant protein

S100A1 is a Ca2+ binding protein of the EF-hand type that belongs to the S100 protein family. S100 proteins consisting of at least 19 members exist as dimers in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell-cycle progression and cell differentiation. This protein has been shown to function in the processes including stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of PKC-mediated phosphorylation. Phosphoglucomutase is a target protein whose activity is antagonistically regulated by S100A1, and recently, S100A1 is also identified as a potent molecular chaperone and a new member of the Hsp70/Hsp90 multichaperone complex. S100A1 displays a tissue-specific expression pattern with highest levels in myocardium and is considered to be an important regulator of cardiac contractility. Accordingly, reduced expression or mutations of S100A1 gene have been implicated in cardiomyopathies.

Product Categories/Family for S100A1 recombinant protein

Calcium-binding Proteins and Related Molecules

References

Remppis, A.et al., 1996, Biochim. Biophs. Acta. 1313: 253-257. Most, P. et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98: 13889-13894 Okada, M. et al., 2004, J. Biol. Chem. 279: 4221-4233. Schafer, W.E. et al., 1995, Genomics. 25: 638-643. Landar, A. et al., 1996, Cell. Calcium. 20: 279-285.

NCBI and Uniprot Product Information

• NCBI GI #: 6755386
• NCBI GeneID: 20193
• NCBI Accession #: NP_035439.1
• NCBI GenBank Nucleotide #: NM_011309.3
• UniProt Accession #: P56565; O88949
• Molecular Weight: 10,505 Da
• NCBI Official Full Name: protein S100-A1
• NCBI Official Synonym Full Names: S100 calcium binding protein A1
• NCBI Official Symbol: S100a1
• NCBI Official Synonym Symbols: S100; S100a; AI266795
• NCBI Protein Information: protein S100-A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 calcium-binding protein A1
• UniProt Protein Name: Protein S100-A1
• Protein Family: Protein
• UniProt Gene Name: S100a1
• UniProt Entry Name: S10A1_MOUSE

Uniprot Description

S100A1: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. Interacts with AGER and CAPZA1. Interacts with FKBP4. Interacts with RYR1 and RYR2. Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. Belongs to the S-100 family.

Protein type: Calcium-binding; Endoplasmic reticulum

Cellular Component: I band; neuron projection; sarcoplasmic reticulum; cytoplasm; M band; nucleus; Z disc; A band

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; metal ion binding; calcium ion binding; calcium-dependent protein binding; ATPase binding

Biological Process: negative regulation of transcription from RNA polymerase II promoter; regulation of heart contraction