Rabbit anti-Mouse S100A1 Polyclonal Antibody | anti-S100A1 antibody

Anti-S100A1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8111293

• Gene Names: S100a1; S100; S100a; AI266795
• Reactivity: Mouse
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: S100A1; Polyclonal Antibody; Anti-S100A1 Antibody; Rabbit Polyclonal; S100A1 Antibody; Rabbit PAb; Antigen Affinity Purified; S100 calcium binding protein A1; Anti-AI266795 Antibody; Anti-S100 Antibody; Anti-S100a Antibody; anti-S100A1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse S100A1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-S100A1 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000
• Immunogen: Recombinant Mouse S100A1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse S100A1 (rM S100A1). S100A1 specific IgG was purified by Mouse S100A1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse S100A1 in mouse uterus with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to uterine gland.)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse S100A1 in mouse heart with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to cardiac muscle.)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse S100A1 in mouse brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Western Blot (WB)

(Anti-S100A1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:11 kDaObserved band size:17 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-S100A1 antibody

S100A1 is a Ca2+ binding protein of the EF-hand type that belongs to the S100 protein family. S100 proteins consisting of at least 19 members exist as dimers in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell-cycle progression and cell differentiation. This protein has been shown to function in the processes including stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of PKC-mediated phosphorylation. Phosphoglucomutase is a target protein whose activity is antagonistically regulated by S100A1, and recently, S100A1 is also identified as a potent molecular chaperone and a new member of the Hsp70/Hsp90 multichaperone complex. S100A1 displays a tissue-specific expression pattern with highest levels in myocardium and is considered to be an important regulator of cardiac contractility. Accordingly, reduced expression or mutations of S100A1 gene have been implicated in cardiomyopathies.

Product Categories/Family for anti-S100A1 antibody

Calcium-binding Proteins and Related Molecules

References

Remppis, A.et al., 1996, Biochim. Biophs. Acta. 1313: 253-257. Most, P. et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98: 13889-13894 Okada, M. et al., 2004, J. Biol. Chem. 279: 4221-4233. Schafer, W.E. et al., 1995, Genomics. 25: 638-643. Landar, A. et al., 1996, Cell. Calcium. 20: 279-285.

NCBI and Uniprot Product Information

• NCBI GI #: 6755386
• NCBI GeneID: 20193
• NCBI Accession #: NP_035439.1
• NCBI GenBank Nucleotide #: NM_011309.3
• UniProt Accession #: P56565; O88949
• Molecular Weight: 10,505 Da
• NCBI Official Full Name: protein S100-A1
• NCBI Official Synonym Full Names: S100 calcium binding protein A1
• NCBI Official Symbol: S100a1
• NCBI Official Synonym Symbols: S100; S100a; AI266795
• NCBI Protein Information: protein S100-A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 calcium-binding protein A1
• UniProt Protein Name: Protein S100-A1
• Protein Family: Protein
• UniProt Gene Name: S100a1
• UniProt Entry Name: S10A1_MOUSE

Uniprot Description

S100A1: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. Interacts with AGER and CAPZA1. Interacts with FKBP4. Interacts with RYR1 and RYR2. Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. Belongs to the S-100 family.

Protein type: Calcium-binding; Endoplasmic reticulum

Cellular Component: I band; neuron projection; sarcoplasmic reticulum; cytoplasm; M band; nucleus; Z disc; A band

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; metal ion binding; calcium ion binding; calcium-dependent protein binding; ATPase binding

Biological Process: negative regulation of transcription from RNA polymerase II promoter; regulation of heart contraction