PTGS2 sirna

PTGS2 siRNA (Mouse)

Cat. Num. AAA8216399

• Gene Names: Ptgs2; COX2; Cox-2; PES-2; PHS-2; Pghs2; TIS10; PGHS-2; PHS II; gripghs
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PTGS2; PTGS2 siRNA (Mouse); COX-2; COX2; PGHS-B; TIS10; Prostaglandin G/H synthase 2; Cyclooxygenase-2; Glucocorticoid-regulated inflammatory cyclooxygenase; Gripghs; Macrophage activation-associated marker protein P71/73; PES-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; PGHS-2; Prostaglandin-endoperoxide synthase 2; TIS10 protein; PTGS2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PTGS2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 604

• Applicable Applications for PTGS2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PTGS2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PTGS2 sirna

siRNA to inhibit PTGS2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31981525
• NCBI GeneID: 19225
• NCBI Accession #: NP_035328.2
• NCBI GenBank Nucleotide #: NM_011198.4
• UniProt Accession #: Q05769; Q543K3
• Molecular Weight: 69,013 Da
• NCBI Official Full Name: prostaglandin G/H synthase 2
• NCBI Official Synonym Full Names: prostaglandin-endoperoxide synthase 2
• NCBI Official Symbol: Ptgs2
• NCBI Official Synonym Symbols: COX2; Cox-2; PES-2; PHS-2; Pghs2; TIS10; PGHS-2; PHS II; gripghs
• NCBI Protein Information: prostaglandin G/H synthase 2
• UniProt Protein Name: Prostaglandin G/H synthase 2
• Protein Family: Prostaglandin G/H synthase
• UniProt Gene Name: Ptgs2
• UniProt Synonym Gene Names: Cox-2; Cox2; Pghs-b; Tis10; COX-2; PGH synthase 2; PGHS-2
• UniProt Entry Name: PGH2_MOUSE

Uniprot Description

COX-2: Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Homodimer. Belongs to the prostaglandin G/H synthase family.

Protein type: Oxidoreductase; Lipid Metabolism - arachidonic acid; EC 1.14.99.1

Cellular Component: neuron projection; protein complex; intracellular membrane-bound organelle; membrane; endoplasmic reticulum; cytoplasm; caveola; nucleus

Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; prostaglandin-endoperoxide synthase activity; peroxidase activity; protein homodimerization activity; enzyme binding; dioxygenase activity; metal ion binding; heme binding; oxidoreductase activity; lipid binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; positive regulation of smooth muscle cell proliferation; response to lipopolysaccharide; positive regulation of NF-kappaB import into nucleus; decidualization; prostaglandin biosynthetic process; sensory perception of pain; fatty acid metabolic process; positive regulation of synaptic transmission, glutamatergic; negative regulation of synaptic transmission, dopaminergic; bone mineralization; negative regulation of cell cycle; negative regulation of cell proliferation; regulation of blood pressure; negative regulation of smooth muscle contraction; positive regulation of cell proliferation; angiogenesis; positive regulation of smooth muscle contraction; inflammatory response; fatty acid biosynthetic process; negative regulation of calcium ion transport; cyclooxygenase pathway; positive regulation of synaptic plasticity; learning; memory; prostaglandin metabolic process; regulation of cell proliferation; keratinocyte differentiation; ovulation; response to cytokine stimulus; positive regulation of vasoconstriction; brown fat cell differentiation; positive regulation of fever; lipid metabolic process; response to oxidative stress; anagen; embryo implantation