OPRM1 sirna

OPRM1 siRNA (Mouse)

Cat. Num. AAA8228558

• Gene Names: Oprm1; mor; Oprm; muOR; MOP-R; MOR-1; M-OR-1; MOR-1O
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: OPRM1; OPRM1 siRNA (Mouse); MOR; OPRM; Mu-type opioid receptor; M-OR-1; MOR-1; OPRM1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: OPRM1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 438

• Applicable Applications for OPRM1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse OPRM1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for OPRM1 sirna

siRNA to inhibit OPRM1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 89001114
• NCBI GeneID: 18390
• NCBI Accession #: NP_001034741.1
• NCBI GenBank Nucleotide #: NM_001039652.2
• UniProt Accession #: P42866; Q4U2P4; Q4U2Q6; Q548C6; Q60768; Q6YC50; Q8CAN5; A1XGX3; A1XGX4; A1YAC3; A1YAC4; A5H7G2
• Molecular Weight: 44,654 Da
• NCBI Official Full Name: mu-type opioid receptor isoform MOR-1C
• NCBI Official Synonym Full Names: opioid receptor, mu 1
• NCBI Official Symbol: Oprm1
• NCBI Official Synonym Symbols: mor; Oprm; muOR; MOP-R; MOR-1; M-OR-1; MOR-1O
• NCBI Protein Information: mu-type opioid receptor
• UniProt Protein Name: Mu-type opioid receptor
• Protein Family: Mu-type opioid receptor
• UniProt Gene Name: Oprm1
• UniProt Synonym Gene Names: Mor; Oprm; M-OR-1; MOR-1
• UniProt Entry Name: OPRM_MOUSE

NCBI Description

This gene encodes the mu opioid receptor which is where drugs such as morphine and other opioids have pharmacological effects. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

Uniprot Description

MOR-1: a Gi-protein-coupled receptor for beta-endorphin, morphine and other opiates. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Ligand-binding inactivates adenylyl cyclase, and activates a variety of G-beta-gamma-dependent pathways including the MAPK and the PI3K/Akt cascades. Two splice-variant isoforms have been described.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral

Cellular Component: dendrite cytoplasm; neuron projection; focal adhesion; integral to plasma membrane; dendrite; integral to membrane; perikaryon; cytosol; lipid raft; membrane; cytoplasm; plasma membrane; sarcolemma

Molecular Function: G-protein coupled receptor activity; voltage-gated calcium channel activity; neuropeptide binding; protein domain specific binding; signal transducer activity; protein binding; opioid receptor activity; G-protein alpha-subunit binding; G-protein beta-subunit binding; filamin binding; beta-endorphin receptor activity

Biological Process: positive regulation of neurogenesis; positive regulation of nitric oxide biosynthetic process; cellular response to stress; negative regulation of nitric oxide biosynthetic process; negative regulation of adenylate cyclase activity; locomotory behavior; behavioral response to ethanol; signal transduction; sensory perception of pain; G-protein coupled receptor protein signaling pathway; synaptic transmission; elevation of cytosolic calcium ion concentration; response to ethanol; neuropeptide signaling pathway; reduction of cytosolic calcium ion concentration; positive regulation of appetite; opioid receptor, adenylate cyclase inhibiting pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of sensory perception of pain; G-protein signaling, adenylate cyclase inhibiting pathway; regulation of excitatory postsynaptic membrane potential; dopamine receptor, adenylate cyclase activating pathway