Western Blot (WB)
(Western blot analysis of extracts of variouscell lines, using CA2 antibody.)
Rabbit CA2 Antibody | anti-CA2 antibody
CA2 Antibody
Gene Names
CA2; CAC; CAII; Car2; CA-II; HEL-76; HEL-S-282
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
CA2; CA2 Antibody; CAC; CAII; Car2; CA-II; anti-CA2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
260
Applicable Applications for anti-CA2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant protein of human CA2
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of variouscell lines, using CA2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of variouscell lines, using CA2 antibody.)
Related Product Information for anti-CA2 antibody
Carbonic anhydrases (CA) are a family of ancient zinc metalloenzymes found in almost all living organisms. All CA can be divided into 3 distinct classes (alpha, beta, and gamma) that evolved independently and have no significant homology in sequence and overall folding. All functional CA catalyze the reversible hydration of CO2 into HCO3-and H+ and contain a zinc atom in the active sites essential for catalysis. There are many isoforms of CA in mammals and they all belong to the alpha class (1, 2). CA2 is a cytosolic member of the alpha class. It is the most widely distributed isoform among the mammalian CAs (1). Defects in CA2 are associated with osteopetrosis and renal tubular acidosis (3-5). Elevated expression of CA2 is observed in patients with Alzheimer's disease and the developing brains of Down syndrome patients (6, 7). CA2 is also overexpressed in Gastrointestinal Stromal Tumors (GISTs) and is considered a useful marker for identification (8). Recently, CA2 was reported to facilitate transporter activity of the monocarboxylate transporter isoform 1 and 4 (MCT1/4) independent of its own catalytic activity (9, 10)
Product Categories/Family for anti-CA2 antibody
References
1. Smith, K.S. et al. (1999) Proc Natl Acad Sci USA 96, 15184-9. 2. Tripp, B.C. et al. (2001) J Biol Chem 276, 48615-8. 3. McMahon, C. et al. (2001) Blood 97, 1947-50. 4. Borthwick, K.J. et al. (2003) J Med Genet 40, 115-21. 5. Lai, L.W. et al. (1998) J Clin Invest 101, 1320-5. 6. Palminiello, S. et al. (2008) Brain Res 1190, 193-205. 7. Jang, B.G. et al. (2010) J Alzheimers Dis 21, 939-45. 8. Parkkila, S. et al. (2010) Mod Pathol 23, 743-50. 9. Becker, H.M. et al. (2005) J Biol Chem 280, 39882-9. 10. Becker, H.M. et al. (2011) Proc Natl Acad Sci USA 108, 3071-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29kDa
NCBI Official Full Name
carbonic anhydrase 2 isoform 1
NCBI Official Synonym Full Names
carbonic anhydrase 2
NCBI Official Symbol
CA2
NCBI Official Synonym Symbols
CAC; CAII; Car2; CA-II; HEL-76; HEL-S-282
NCBI Protein Information
carbonic anhydrase 2
UniProt Protein Name
Carbonic anhydrase 2
Protein Family
UniProt Gene Name
CA2
UniProt Synonym Gene Names
CAC; CA-II
NCBI Description
The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
Uniprot Description
Essential for bone resorption and osteoclast differentiation (). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.
Research Articles on CA2
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Product Notes
The CA2 ca2 (Catalog #AAA8502538) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CA2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the CA2 ca2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CA2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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