MYD88 sirna

MYD88 siRNA (Mouse)

Cat. Num. AAA8227447

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYD88; MYD88 siRNA (Mouse); Myeloid differentiation primary response protein MyD88; MYD88 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MYD88 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 250

• Applicable Applications for MYD88 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MYD88 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYD88 sirna

siRNA to inhibit MYD88 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6754772
• NCBI GeneID: 17874
• NCBI Accession #: NP_034981.1
• NCBI GenBank Nucleotide #: NM_010851.2
• UniProt Accession #: P22366; O35916
• Molecular Weight: 28,683 Da
• NCBI Official Full Name: myeloid differentiation primary response protein MyD88
• NCBI Official Synonym Full Names: myeloid differentiation primary response gene 88
• NCBI Official Symbol: Myd88
• NCBI Protein Information: myeloid differentiation primary response protein MyD88
• UniProt Protein Name: Myeloid differentiation primary response protein MyD88
• Protein Family: Myeloid differentiation primary response protein
• UniProt Gene Name: Myd88
• UniProt Entry Name: MYD88_MOUSE

Uniprot Description

MYD88: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1, IRAK2 and IRAK4 via their respective TIR domains. Interacts with IL18R1. Interacts with BMX, IL1RL1 and IRF7. Interacts with LRRFIP1 and LRRFIP2; this interaction positively regulates Toll-like receptor (TLR) signaling in response to agonist. Interacts with FLII. LRRFIP1 and LRRFIP2 compete with FLII for MYD88-binding. Interacts with IRF1. Ubiquitous. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Cellular Component: protein complex; postsynaptic density; cytoplasm; plasma membrane; intracellular; cytosol

Molecular Function: identical protein binding; protein binding; interleukin-1 receptor binding; receptor binding; Toll binding

Biological Process: positive regulation of interleukin-23 production; positive regulation of smooth muscle cell proliferation; positive regulation of JNK cascade; response to lipopolysaccharide; response to molecule of fungal origin; signal transduction; activation of NF-kappaB transcription factor; cell surface receptor linked signal transduction; positive regulation of lymphocyte proliferation; response to peptidoglycan; transmembrane receptor protein serine/threonine kinase signaling pathway; lipopolysaccharide-mediated signaling pathway; JNK cascade; interferon type I biosynthetic process; inflammatory response; positive regulation of interleukin-17 production; positive regulation of I-kappaB kinase/NF-kappaB cascade; immune system process; cytokine and chemokine mediated signaling pathway; response to virus; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; Toll signaling pathway; regulation of cell proliferation; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; positive regulation of chemokine biosynthetic process; regulation of inflammatory response; innate immune response; immune response; immunoglobulin mediated immune response