Western Blot (WB)
(Anti-GAS2 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 whole cell lysate Lane B: HeLa whole cell lysate Lane C: Jurkat whole cell lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:35 kDa (We are unsure as to the identity of these extra bands.))
Rabbit anti-Human GAS2 Polyclonal Antibody | anti-GAS2 antibody
Anti-GAS2 Antibody, Rabbit Polyclonal
IP: 1-2uL/mg of lysate
Western Blot (WB)
(Anti-GAS2 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 whole cell lysate Lane B: HeLa whole cell lysate Lane C: Jurkat whole cell lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:35 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-GAS2 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 whole cell lysate Lane B: HeLa whole cell lysate Lane C: Jurkat whole cell lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:35 kDa (We are unsure as to the identity of these extra bands.))
Immunoprecipitation (IP)
(GAS2 was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate 1 uL anti-GAS2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-GAS2 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size: 35 kDa)
Immunoprecipitation (IP)
(GAS2 was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate 1 uL anti-GAS2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-GAS2 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size: 35 kDa)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a caspase-3 substrate that plays a role in regulating microfilament and cell shape changes during apoptosis. It can also modulate cell susceptibility to p53-dependent apoptosis by inhibiting calpain activity. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2009]
Uniprot Description
GAS2: May play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process. Belongs to the GAS2 family.
Protein type: Motility/polarity/chemotaxis; Apoptosis
Chromosomal Location of Human Ortholog: 11p14.3
Cellular Component: membrane; actin filament; cytosol
Biological Process: regulation of cell shape; apoptosis; cell cycle arrest; cell structure disassembly during apoptosis
Research Articles on GAS2
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Product Notes
The GAS2 gas2 (Catalog #AAA8100801) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GAS2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GAS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:1000 IP: 1-2uL/mg of lysate. Researchers should empirically determine the suitability of the GAS2 gas2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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