MDM2 sirna

MDM2 siRNA (Mouse)

Cat. Num. AAA8206708

• Gene Names: Mdm2; Mdm-2; AA415488; 1700007J15Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MDM2; MDM2 siRNA (Mouse); E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Oncoprotein Mdm2; p53-binding protein Mdm2; MDM2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MDM2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 440

• Applicable Applications for MDM2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MDM2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MDM2 sirna

siRNA to inhibit MDM2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 570700841
• NCBI GeneID: 17246
• NCBI Accession #: NP_001275515.1
• NCBI GenBank Nucleotide #: NM_001288586.1
• UniProt Accession #: P23804; Q61040; Q64330; Q91XK7
• Molecular Weight: 49,351 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase Mdm2
• NCBI Official Synonym Full Names: transformed mouse 3T3 cell double minute 2
• NCBI Official Symbol: Mdm2
• NCBI Official Synonym Symbols: Mdm-2; AA415488; 1700007J15Rik
• NCBI Protein Information: E3 ubiquitin-protein ligase Mdm2
• UniProt Protein Name: E3 ubiquitin-protein ligase Mdm2
• Protein Family: Mdm2-binding protein
• UniProt Gene Name: Mdm2
• UniProt Entry Name: MDM2_MOUSE

Uniprot Description

MDM2: a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 via Akt-mediated MDM2 phosphorylation. Phosphorylation of MDM2 increases its interaction with p300, providing a platform to allow the assembly of the protein complex necessary for MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. Facilitates the nuclear export of p53 and targets it for proteasome-mediated proteolysis. Eight alternatively spliced isoforms have been reported.

Protein type: Oncoprotein; Ubiquitin ligase; Nucleolus; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Inhibitor; EC 6.3.2.-

Cellular Component: nucleoplasm; nuclear body; protein complex; cytoplasm; plasma membrane; nucleolus; synapse; cytosol; nucleus

Molecular Function: identical protein binding; peroxisome proliferator activated receptor binding; zinc ion binding; p53 binding; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; ubiquitin-protein ligase activity; ribosomal S6-glutamic acid ligase activity; protein binding; enzyme binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ubiquitin protein ligase binding; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity

Biological Process: traversing start control point of mitotic cell cycle; protein destabilization; protein ubiquitination during ubiquitin-dependent protein catabolic process; positive regulation of cell cycle; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; positive regulation of mitotic cell cycle; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; positive regulation of protein export from nucleus; negative regulation of DNA damage response, signal transduction by p53 class mediator; establishment of protein localization; peptidyl-lysine modification; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein catabolic process; protein complex assembly; regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis