Rabbit anti-Human KIAA0999 Polyclonal Antibody | anti-KIAA0999 antibody

KIAA0999, CT (SIK3, KIAA0999, QSK, Serine/threonine-protein kinase SIK3, Salt-inducible kinase 3, Serine/threonine-protein kinase QSK) (MaxLight 550)

Cat. Num. AAA6313090

• Gene Names: SIK3; L19; QSK; SIK-3
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: KIAA0999; Polyclonal Antibody; CT (SIK3; QSK; Serine/threonine-protein kinase SIK3; Salt-inducible kinase 3; Serine/threonine-protein kinase QSK) (MaxLight 550); SIK3; Serine/threonine-protein kinase QSK; anti-KIAA0999 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KIAA0999.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-KIAA0999 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1233-1263 in the C-terminal region of human KIAA0999.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Hela cell line lysates (35ug/lane) usingMBS6001296. This demonstrates that MBS6001296 detected the KIAA0999 protein (arrow).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Hela cells using MBS6001296 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-KIAA0999 antibody

MaxLight550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor546, 555, DyLight549, Cy3, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Product Categories/Family for anti-KIAA0999 antibody

Antibodies; Protein Kinases

References

1. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010). 2. Couch, F.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(1):251-257(2010). 3. Huang, C.J., et al. J. Cell. Mol. Med. 12 (5B), 1936-1943 (2008). 4. Hashimoto, Y.K., et al. J. Cell. Biochem. 104(5):1724-1739(2008). 5. Kooner, J.S., et al. Nat. Genet. 40(2):149-151(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 38569491
• NCBI GeneID: 23387
• NCBI Accession #: NP_079440.2
• NCBI GenBank Nucleotide #: NM_025164.3
• UniProt Accession #: Q9Y2K2; Q59FY2; Q5M9N1; Q6P3R6; Q8IYM8; Q9HA50
• Molecular Weight: 139,980 Da
• NCBI Official Full Name: serine/threonine-protein kinase SIK3
• NCBI Official Synonym Full Names: SIK family kinase 3
• NCBI Official Symbol: SIK3
• NCBI Official Synonym Symbols: L19; QSK; SIK-3
• NCBI Protein Information: serine/threonine-protein kinase SIK3; salt-inducible kinase 3; serine/threonine-protein kinase QSK
• UniProt Protein Name: Serine/threonine-protein kinase SIK3
• UniProt Gene Name: SIK3
• UniProt Synonym Gene Names: KIAA0999; QSK; SIK-3
• UniProt Entry Name: SIK3_HUMAN

Uniprot Description

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Enzyme regulation: Activated by phosphorylation on Thr-163

By similarity. Ref.1

Subunit structure: Binds to and is activated by YWHAZ when phosphorylated on Thr-163. Ref.8

Subcellular location: Cytoplasm. Note: Locates to punctate structures within the cytoplasm on binding to YWHAZ. Ref.8

Post-translational modification: Phosphorylated at Thr-163 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Ref.1

Sequence similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.Contains 1 protein kinase domain.Contains 1 UBA domain.

Sequence caution: The sequence AAT06749.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA76843.2 differs from that shown. Reason: Erroneous initiation. The sequence BAB14006.1 differs from that shown. Reason: Erroneous initiation.