Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse E3 ubiquitin-protein ligase Mdm2 (MDM2) Standard Curve.)
Mouse E3 Ubiquitin-Protein Ligase Mdm2 (MDM2) ELISA Kit | MDM2 elisa kit
Mouse E3 Ubiquitin-Protein Ligase Mdm2 (MDM2) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse E3 ubiquitin-protein ligase Mdm2 (MDM2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse E3 ubiquitin-protein ligase Mdm2 (MDM2) Standard Curve.)
Background: MDM2 encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. MDM2 is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for MDM2 on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
NCBI and Uniprot Product Information
Uniprot Description
MDM2: a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 via Akt-mediated MDM2 phosphorylation. Phosphorylation of MDM2 increases its interaction with p300, providing a platform to allow the assembly of the protein complex necessary for MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. Facilitates the nuclear export of p53 and targets it for proteasome-mediated proteolysis. Eight alternatively spliced isoforms have been reported.
Protein type: Ligase; Ubiquitin conjugating system; Ubiquitin ligase; Inhibitor; Oncoprotein; EC 6.3.2.19; Nucleolus; EC 6.3.2.-
Cellular Component: nucleoplasm; nuclear body; protein complex; cytoplasm; plasma membrane; nucleolus; synapse; cytosol; nucleus
Molecular Function: identical protein binding; peroxisome proliferator activated receptor binding; zinc ion binding; p53 binding; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; ubiquitin-protein ligase activity; ribosomal S6-glutamic acid ligase activity; protein binding; enzyme binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ubiquitin protein ligase binding; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity
Biological Process: traversing start control point of mitotic cell cycle; protein destabilization; protein ubiquitination during ubiquitin-dependent protein catabolic process; positive regulation of cell cycle; protein ubiquitination; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; positive regulation of protein export from nucleus; negative regulation of DNA damage response, signal transduction by p53 class mediator; establishment of protein localization; peptidyl-lysine modification; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein complex assembly; protein catabolic process; regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
Research Articles on MDM2
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Product Notes
The Mouse MDM2 mdm2 (Catalog #AAA9717689) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717689 ELISA Kit recognizes Mouse MDM2. It is sometimes possible for the material contained within the vial of "E3 Ubiquitin-Protein Ligase Mdm2 (MDM2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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