Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2 antibody (Ser166))
Rabbit MDM2 Polyclonal Antibody | anti-MDM2 antibody
MDM2 Antibody (Ser166)
Gene Names
MDM2; HDMX; hdm2; ACTFS
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Chromatography
Synonyms
MDM2; Polyclonal Antibody; MDM2 Antibody (Ser166); Rabbit Polyclonal MDM2 Antibody (Ser166); Polyclonal MDM2 antibody; Anti-MDM2 antibody; 1700007J15Rik antibody; AA415488 antibody; Double minute 2 protein antibody; E3 ubiquitin-protein ligase Mdm2 antibody; Mdm-2 antibody; Mdm2 antibody; Oncoprotein Mdm2 antibody; OTTMUSP00000022916 antibody; p53-binding protein Mdm2 antibody; transformed mouse 3T3 cell double minute 2 antibody; anti-MDM2 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
MDM2 antibody (Ser166) detects endogenous levels of MDM2 only whenphosphorylated at Serine 166
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
257
Applicable Applications for anti-MDM2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:200
IHC: 1:50-1:100
IF: 1:100-1:200
Immunogen
MDM2 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human MDM2 around the phosphorylation site of Serine 166 (A-I-SP-E-T) as the immunogen.
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2 antibody (Ser166))
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2 antibody (Ser166))
Western Blot (WB)
(Western blot analysis of extracts from HT29 cell using MDM2 (phospho-Ser166) antibody)
Western Blot (WB)
(Western blot analysis of extracts from HT29 cell using MDM2 (phospho-Ser166) antibody)
Related Product Information for anti-MDM2 antibody
MDM2 is an ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 via Akt-mediated MDM2 phosphorylation. Phosphorylation of MDM2 increases its interaction with p300, providing a platform to allow the assembly of the protein complex necessary for MDM2-mediated ubiquitination and degradation of p53.
Product Categories/Family for anti-MDM2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Mdm2, partial
NCBI Official Synonym Full Names
MDM2 proto-oncogene
NCBI Official Symbol
MDM2
NCBI Official Synonym Symbols
HDMX; hdm2; ACTFS
NCBI Protein Information
E3 ubiquitin-protein ligase Mdm2
Protein Family
NCBI Description
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]
Research Articles on MDM2
Similar Products
Product Notes
The MDM2 (Catalog #AAA5313752) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MDM2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:1000 IHC: 1:50-1:100 IF: 1:100-1:200. Researchers should empirically determine the suitability of the MDM2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MDM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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