ITGAV sirna

ITGAV siRNA (Mouse)

Cat. Num. AAA8200430

• Gene Names: Itgav; CD51; 1110004F14Rik; 2610028E01Rik; D430040G12Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ITGAV; ITGAV siRNA (Mouse); Integrin alpha-V; Vitronectin receptor subunit alpha; CD antigen CD51; ITGAV sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ITGAV siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1044

• Applicable Applications for ITGAV sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ITGAV gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ITGAV sirna

siRNA to inhibit ITGAV expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 154240716
• NCBI GeneID: 16410
• NCBI Accession #: NP_032428.2
• NCBI GenBank Nucleotide #: NM_008402.3
• UniProt Accession #: P43406; A2AKI6
• Molecular Weight: 115,360 Da
• NCBI Official Full Name: integrin alpha-V
• NCBI Official Synonym Full Names: integrin alpha V
• NCBI Official Symbol: Itgav
• NCBI Official Synonym Symbols: CD51; 1110004F14Rik; 2610028E01Rik; D430040G12Rik
• NCBI Protein Information: integrin alpha-V
• UniProt Protein Name: Integrin alpha-V
• Protein Family: Integrin
• UniProt Gene Name: Itgav
• UniProt Entry Name: ITAV_MOUSE

NCBI Description

This gene encodes a protein that is a member of the integrin superfamily. Integrins are transmembrane receptors involved cell adhesion and signaling, and they are subdivided based on the heterodimer formation of alpha and beta chains. This protein has been shown to heterodimerize with beta 1, beta 3, beta 6 and beta 8. The heterodimer of alpha v and beta 3 forms the Vitronectin receptor. This protein interacts with several extracellular matrix proteins to mediate cell adhesion and may play a role in cell migration. In mouse, deficiency of this gene is associated with defects in vascular morphogenesis in the brain and early post-natal death. [provided by RefSeq, May 2013]

Uniprot Description

ITGAV: The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Belongs to the integrin alpha chain family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: filopodium membrane; focal adhesion; cell surface; membrane; microvillus membrane; plasma membrane; integral to membrane; intracellular; nucleus; cytosol; integrin complex; external side of plasma membrane

Molecular Function: voltage-gated calcium channel activity; opsonin binding; insulin-like growth factor I binding; protease binding; protein kinase C binding; extracellular matrix binding; protein heterodimerization activity; fibronectin binding; metal ion binding; peptide binding; receptor binding

Biological Process: negative regulation of lipid transport; focal adhesion formation; entry of virus into host cell; positive regulation of cell adhesion; T cell activation; cell-matrix adhesion; multicellular organismal development; negative chemotaxis; gonad morphogenesis; regulation of phagocytosis; elevation of cytosolic calcium ion concentration; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; angiogenesis; cell growth; inflammatory response; cell adhesion; cell differentiation; integrin-mediated signaling pathway; blood vessel development; regulation of bone resorption; cell migration; negative regulation of lipoprotein metabolic process; detection of molecule of bacterial origin; cell-substrate adhesion; cellular calcium ion homeostasis; heterotypic cell-cell adhesion; fertilization; negative regulation of low-density lipoprotein receptor biosynthetic process; release of sequestered calcium ion into cytosol; positive regulation of osteoblast proliferation; endothelial cell migration; apoptotic cell clearance; positive regulation of cell migration