ICOS sirna

ICOS siRNA (Mouse)

Cat. Num. AAA8239853

• Gene Names: Icos; H4; CCLP; AILIM; CRP-1; Ly115
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ICOS; ICOS siRNA (Mouse); AILIM; Inducible T-cell costimulator; Activation-inducible lymphocyte immunomediatory molecule; CD28 and CTLA-4-like protein; CCLP; CD28-related protein 1; CRP-1; CD antigen CD278; ICOS sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ICOS siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 200

• Applicable Applications for ICOS sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ICOS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ICOS sirna

siRNA to inhibit ICOS expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 224809335
• NCBI GeneID: 54167
• NCBI Accession #: NP_059508.2
• NCBI GenBank Nucleotide #: NM_017480.2
• UniProt Accession #: Q9WVS0; Q9JL17
• Molecular Weight: 22,709 Da
• NCBI Official Full Name: inducible T-cell costimulator
• NCBI Official Synonym Full Names: inducible T cell co-stimulator
• NCBI Official Symbol: Icos
• NCBI Official Synonym Symbols: H4; CCLP; AILIM; CRP-1; Ly115
• NCBI Protein Information: inducible T-cell costimulator
• UniProt Protein Name: Inducible T-cell costimulator
• Protein Family: ICOS ligand
• UniProt Gene Name: Icos
• UniProt Synonym Gene Names: Ailim; CCLP; CRP-1
• UniProt Entry Name: ICOS_MOUSE

Uniprot Description

ICOS: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up- regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up- regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre- activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. Defects in ICOS are the cause of immunodeficiency common variable type 1 (CVID1). CVID1 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be lo. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding

Biological Process: cell-cell adhesion; T cell tolerance induction; T cell costimulation