ICOS active protein

ICOS Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122763

• Gene Names: Icos; H4; CCLP; AILIM; CRP-1; Ly115
• Purity: >90% as determined by SDS-PAGE
• Synonyms: ICOS; ICOS Protein; Mouse; Recombinant (His Tag); Mouse ICOS Protein (His Tag); AILIM Protein; CCLP Protein; CRP-1 Protein; H4 Protein; Ly115 Protein; inducible T-cell co-stimulator; ICOS active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln135

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Glu 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse ICOS (NP_034643.2)(Met1-Gln135) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized Rat ICOS ligand hFc at 2ug/mL (100uL/well) can bind Mouse ICOS His. The EC50 of Mouse ICOS His is 150-800ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized Rat ICOS ligand hFc at 2 ug/mL (100 uL/well) can bind Mouse ICOS His, the EC50 of Mouse ICOS His is 150-800 ng/mL.)

Related Product Information for ICOS active protein

Background: Inducible costimulator (ICOS), also called AILIM (Activation-Inducible Lymphocyte Immunomediatory Molecule) is a cell-surface receptor and belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4, and PD-1. The interaction of B7-H2/ICOS plays a critical role in Th cell differentiation, T-B cell interactions which are essential for the germinal center formation, and humoral immune responses, and as well as the production of cytokine IL-4. Also, ICOS is more potent in the induction of IL-10 production, a cytokine important for the suppressive function of T regulatory cells. The B7-1/B7-2--CD28/CTLA-4 and ICOS-B7RP-1 pathway provide key second signals that can regulate the activation, inhibition, and fine-tuning of T-lymphocyte responses. ICOS stimulates both Th1 and Th2 cytokine production but may have a preferential role in Th2 cell development. Moreover, The B7-1/B7-2-CD28/CTLA-4 and ICOS-B7RP-1 pathway has been suggested as being involved in the development of airway inflammation and airway hyperresponsiveness.

Product Categories/Family for ICOS active protein

Cancer Drug Targets

References

Coyle AJ, et al. (2004) The role of ICOS and other costimulatory molecules in allergy and asthma. Springer Semin Immunopathol. 25(3-4): 349-59.Chen YQ, et al. (2006) CD28/CTLA-4--CD80/CD86 and ICOS--B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61(1): 15-26.van Berkel ME, et al. (2006) CD28 and ICOS: similar or separate costimulators of T cells Immunol Lett. 105(2): 115-22.

NCBI and Uniprot Product Information

• NCBI GI #: 224809335
• NCBI GeneID: 54167
• NCBI Accession #: NP_059508.2
• NCBI GenBank Nucleotide #: NM_017480.2
• UniProt Accession #: Q9WVS0; Q9JL17
• Molecular Weight: 22,709 Da
• NCBI Official Full Name: inducible T-cell costimulator
• NCBI Official Synonym Full Names: inducible T cell co-stimulator
• NCBI Official Symbol: Icos
• NCBI Official Synonym Symbols: H4; CCLP; AILIM; CRP-1; Ly115
• NCBI Protein Information: inducible T-cell costimulator
• UniProt Protein Name: Inducible T-cell costimulator
• Protein Family: ICOS ligand
• UniProt Gene Name: Icos
• UniProt Synonym Gene Names: Ailim; CCLP; CRP-1

Uniprot Description

Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes ().