HPGD sirna

HPGD siRNA (Mouse)

Cat. Num. AAA8220686

• Gene Names: Hpgd; 15-PGDH; AV026552
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HPGD; HPGD siRNA (Mouse); PGDH1; 15-hydroxyprostaglandin dehydrogenase [NAD(+)]; 15-PGDH; Prostaglandin dehydrogenase 1; HPGD sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: HPGD siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 269

• Applicable Applications for HPGD sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HPGD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HPGD sirna

siRNA to inhibit HPGD expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 124486706
• NCBI GeneID: 15446
• NCBI Accession #: NP_032304.2
• NCBI GenBank Nucleotide #: NM_008278.2
• UniProt Accession #: Q8VCC1; Q61106
• Molecular Weight: 29,181 Da
• NCBI Official Full Name: 15-hydroxyprostaglandin dehydrogenase
• NCBI Official Synonym Full Names: hydroxyprostaglandin dehydrogenase 15 (NAD)
• NCBI Official Symbol: Hpgd
• NCBI Official Synonym Symbols: 15-PGDH; AV026552
• NCBI Protein Information: 15-hydroxyprostaglandin dehydrogenase [NAD(+)]
• UniProt Protein Name: 15-hydroxyprostaglandin dehydrogenase [NAD(+)]
• Protein Family: 15-hydroxyprostaglandin dehydrogenase
• UniProt Gene Name: Hpgd
• UniProt Synonym Gene Names: Pgdh1; 15-PGDH
• UniProt Entry Name: PGDH_MOUSE

Uniprot Description

HPGD: Prostaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. Inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of hypertrophic osteoarthropathy, primary, autosomal recessive, type 1 (PHOAR1). A disease characterized by digital clubbing, periostosis, acroosteolysis, painful joint enlargement, and variable features of pachydermia that include thickened facial skin and a thickened scalp. Other developmental anomalies include delayed closure of the cranial sutures and congenital heart disease. Defects in HPGD are the cause of cranioosteoarthropathy (COA). A form of osterarthropathy characterized by swelling of the joints, digital clubbing, hyperhidrosis, delayed closure of the fontanels, periostosis, and variable patent ductus arteriosus. Pachydermia is not a prominent feature. Defects in HPGD are a cause of isolated congenital nail clubbing (ICNC); also called clubbing of digits or hereditary acropachy. ICNC is a rare genodermatosis characterized by enlargement of the nail plate and terminal segments of the fingers and toes, resulting from proliferation of the connective tissues between the nail matrix and the distal phalanx. It is usually symmetrical and bilateral (in some cases unilateral). In nail clubbing usually the distal end of the nail matrix is relatively high compared to the proximal end, while the nail plate is complete but its dimensions and diameter more or less vary in comparison to normal. There may be different fingers and toes involved to varying degrees. Some fingers or toes are spared, but the thumbs are almost always involved. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.141; Oxidoreductase; Tumor suppressor

Cellular Component: basolateral plasma membrane; cytoplasm

Molecular Function: 15-hydroxyprostaglandin dehydrogenase (NAD+) activity; oxidoreductase activity; NAD binding; catalytic activity; prostaglandin E receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; metabolic process; transforming growth factor beta receptor signaling pathway; lipid metabolic process; parturition; female pregnancy; fatty acid metabolic process; negative regulation of cell cycle; prostaglandin metabolic process