GOT2 active protein

GOT2, 30-430aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA206319

• Gene Names: GOT2; KAT4; KATIV; KYAT4; mitAAT
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: GOT2; 30-430aa; Human; His tag; E Coli (Bioactivity Validated); Aspartate aminotransferase 2 mitochondrial; KAT4; KATIV; mitAAT; GOT2 active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSSSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK
• Sequence Length: 430

• Applicable Applications for GOT2 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Specific activity is > 60 units/mg, and is defined as the amount of enzyme that convert 1umole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.
• Assay: 1. Prepare a 180ul assay buffer into a suitable container. The concentrations are 110mM Tris-HCl (pH8.0), 0.44mM beta-nicotinamide adenine dinucleotide, reduced form, 55mM L-aspartate, 2.75mM alpha-ketoglutaric acid, 82.5 units MDH.; 2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer.; 3. Add 20ul of recombinant GOT2 protein 2ug/ml in assay buffer.; 4. Mix by inversion and record the decrease at A340nm for approximately 5 minutes.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GOT2 active protein

GOT2, also known as glutamate oxaloacetate transaminase 2, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. glutamate oxaloacetate transaminas is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for GOT2 active protein

Metabolism

References

Guidetti P, Amori L, et al. (2007). J Neurochem. 102 (1):103-11.; Lain B, Iriarte A, et al. (1995). J Biol Chem. 270 (42):24732-9.

NCBI and Uniprot Product Information

• NCBI GI #: 73486658
• NCBI GeneID: 2806
• NCBI Accession #: NP_002071
• NCBI GenBank Nucleotide #: NM_002080.3
• UniProt Accession #: P00505; Q53FL3; Q9BWA3; B4DJA6; E7ERW2
• Molecular Weight: 47 kDa (424aa), confirmed by MALDI-TOF
• NCBI Official Full Name: aspartate aminotransferase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: glutamic-oxaloacetic transaminase 2
• NCBI Official Symbol: GOT2
• NCBI Official Synonym Symbols: KAT4; KATIV; KYAT4; mitAAT
• NCBI Protein Information: aspartate aminotransferase, mitochondrial
• UniProt Protein Name: Aspartate aminotransferase, mitochondrial
• UniProt Gene Name: GOT2
• UniProt Synonym Gene Names: mAspAT; FABP-1; FABPpm

NCBI Description

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Uniprot Description

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.