GYG1 sirna

GYG1 siRNA (Mouse)

Cat. Num. AAA8205186

• Gene Names: Gyg; Gyg1; AU017667
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GYG1; GYG1 siRNA (Mouse); GYG; Glycogenin-1; GN-1; GN1; GYG1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GYG1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 333

• Applicable Applications for GYG1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GYG1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GYG1 sirna

siRNA to inhibit GYG1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7305121
• NCBI GeneID: 27357
• NCBI Accession #: NP_038783.1
• NCBI GenBank Nucleotide #: NM_013755.3
• UniProt Accession #: Q9R062; Q3TWR9
• Molecular Weight: 37,402 Da
• NCBI Official Full Name: glycogenin-1
• NCBI Official Synonym Full Names: glycogenin
• NCBI Official Symbol: Gyg
• NCBI Official Synonym Symbols: Gyg1; AU017667
• NCBI Protein Information: glycogenin-1
• UniProt Protein Name: Glycogenin-1
• UniProt Gene Name: Gyg1
• UniProt Synonym Gene Names: Gyg; GN-1; GN1
• UniProt Entry Name: GLYG_MOUSE

Uniprot Description

glycogenin: Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase. Defects in GYG1 are the cause of glycogen storage disease type 15 (GSD15). It is a metabolic disorder resulting in muscle weakness, associated with the glycogen depletion in skeletal muscle, and cardiac arrhythmia, associated with the accumulation of abnormal storage material in the heart. The skeletal muscle shows a marked predominance of slow-twitch, oxidative muscle fibers and mitochondrial proliferation. Belongs to the glycosyltransferase 8 family. Glycogenin subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.4.1.186; Transferase

Cellular Component: membrane

Molecular Function: transferase activity; transferase activity, transferring glycosyl groups; metal ion binding; glycogenin glucosyltransferase activity; glucose binding

Biological Process: glycogen biosynthetic process