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Western Blot (WB) (Western blot analysis of KLRD1 Antibody (N-term) in K562 cell line lysates (35ug/lane). KLRD1 (arrow) was detected using the purified Pab.)

Rabbit anti-Human CD94 Polyclonal Antibody | anti-CD94 antibody

CD94, NT (CD94 Antigen, Killer Cell Lectin-like Receptor Subfamily D Member 1, KLRD1, KLRD1 Protein, KP43, Natural Killer Cells Antigen CD94, NK Cell Receptor) (AP)

Gene Names
KLRD1; CD94
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Immunoaffinity chromatography.
Synonyms
CD94; Polyclonal Antibody; NT (CD94 Antigen; Killer Cell Lectin-like Receptor Subfamily D Member 1; KLRD1; KLRD1 Protein; KP43; Natural Killer Cells Antigen CD94; NK Cell Receptor) (AP); anti-CD94 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human KLRD1.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Sequence Length
179
Applicable Applications for anti-CD94 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-1:500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-term region of human KLRD1 (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KLRD1 Antibody (N-term) in K562 cell line lysates (35ug/lane). KLRD1 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of KLRD1 Antibody (N-term) in K562 cell line lysates (35ug/lane). KLRD1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lymph with KLRD1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lymph with KLRD1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of CEM cells using KLRD1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of CEM cells using KLRD1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-CD94 antibody
References
1. Chang, C., et.al., Eur. J. Immunol. 25(9), 2433-2437 (1995). 2. Rodriguez, A., et.al., Immunogenetics 47(4), 305-309 (1998).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20.497
NCBI Official Full Name
natural killer cells antigen CD94 isoform 1
NCBI Official Synonym Full Names
killer cell lectin like receptor D1
NCBI Official Symbol
KLRD1
NCBI Official Synonym Symbols
CD94
NCBI Protein Information
natural killer cells antigen CD94
UniProt Protein Name
Natural killer cells antigen CD94
UniProt Gene Name
KLRD1
UniProt Synonym Gene Names
CD94
UniProt Entry Name
KLRD1_HUMAN

NCBI Description

Natural killer (NK) cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2017]

Uniprot Description

KLRD1: Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: integral to membrane; plasma membrane; receptor complex; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; carbohydrate binding

Biological Process: regulation of immune response; cell surface receptor linked signal transduction; natural killer cell mediated immunity; innate immune response

Research Articles on CD94

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Product Notes

The CD94 klrd1 (Catalog #AAA6468159) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD94, NT (CD94 Antigen, Killer Cell Lectin-like Receptor Subfamily D Member 1, KLRD1, KLRD1 Protein, KP43, Natural Killer Cells Antigen CD94, NK Cell Receptor) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD94 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-1:500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CD94 klrd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD94, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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