FXYD2 sirna

FXYD2 siRNA (Mouse)

Cat. Num. AAA829427

• Gene Names: Fxyd2; Atp1g1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FXYD2; FXYD2 siRNA (Mouse); ATP1C; ATP1G1; Sodium/potassium-transporting ATPase subunit gamma; Na(+)/K(+) ATPase subunit gamma; FXYD domain-containing ion transport regulator 2; Sodium pump gamma chain; FXYD2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FXYD2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 70

• Applicable Applications for FXYD2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FXYD2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FXYD2 sirna

siRNA to inhibit FXYD2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16554570
• NCBI GeneID: 11936
• NCBI Accession #: NP_031529.2
• NCBI GenBank Nucleotide #: NM_007503.3
• UniProt Accession #: Q04646; Q923I2; Q923I3; Q923I4
• Molecular Weight: 11,281 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit gamma isoform a
• NCBI Official Synonym Full Names: FXYD domain-containing ion transport regulator 2
• NCBI Official Symbol: Fxyd2
• NCBI Official Synonym Symbols: Atp1g1
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit gamma
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit gamma
• Protein Family: Sodium/potassium-transporting ATPase
• UniProt Gene Name: Fxyd2
• UniProt Synonym Gene Names: Atp1c; Atp1g1; Na(+)/K(+) ATPase subunit gamma
• UniProt Entry Name: ATNG_MOUSE

NCBI Description

This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The Type III integral membrane protein encoded by this gene is the gamma subunit of the Na,K-ATPase present on the plasma membrane. Although the Na,K-ATPase does not depend on the gamma subunit to be functional, it is thought that the gamma subunit modulates the enzyme's activity by inducing ion channel activity. Multiple transcript variants have been described for this gene that are expressed in tissue-specific and developmental stage-specific patterns and encode proteins that differ at the N-terminus. [provided by RefSeq, Sep 2009]

Uniprot Description

FXYD2: May be involved in forming the receptor site for cardiac glycoside binding or may modulate the transport function of the sodium ATPase. Defects in FXYD2 are the cause of hypomagnesemia type 2 (HOMG2); also known as dominant renal hypomagnesemia or hypomagnesemia with hypocalciuria. HOMG2 is a disorder due to primary renal wasting of magnesium. Plasma levels of other electrolytes are normal. The only abnormality found, in addition to low magnesium levels, is lowered renal excretion of calcium resulting in hypocalciuria. Belongs to the FXYD family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to plasma membrane; integral to membrane

Molecular Function: sodium channel regulator activity; ion channel activity

Biological Process: transport; sodium ion transport; ion transport; potassium ion transport