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Testing Data #2 (Western blot analysis of extracts from NIH/3T3 cells, A549 cells and HT-29 cells, using ATP2A1 antibody.)

ATP2A1 Antibody | anti-ATP2A1 antibody

ATP2A1 Antibody

Gene Names
ATP2A1; ATP2A; SERCA1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
ATP2A1; ATP2A1 Antibody; EC 3.6.3.8; Calcium pump 1; SERCA1; SR Ca(2+)-ATPase 1; Calcium-transporting ATPase sarcoplasmic reticulum type; fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; anti-ATP2A1 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
ATP2A1 antibody detects endogenous levels of total ATP2A1 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
869
Applicable Applications for anti-ATP2A1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human ATP2A1.
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data #2

(Western blot analysis of extracts from NIH/3T3 cells, A549 cells and HT-29 cells, using ATP2A1 antibody.)

Testing Data #2 (Western blot analysis of extracts from NIH/3T3 cells, A549 cells and HT-29 cells, using ATP2A1 antibody.)
References
Zhang Y., Genomics 30:415-424(1995). Ota T., Nat. Genet. 36:40-45(2004). Daiho T., J. Biol. Chem. 276:32771-32778(2001).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
487
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
95,199 Da
NCBI Official Full Name
sarcoplasmic/endoplasmic reticulum calcium ATPase 1 isoform c
NCBI Official Synonym Full Names
ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1
NCBI Official Symbol
ATP2A1
NCBI Official Synonym Symbols
ATP2A; SERCA1
NCBI Protein Information
sarcoplasmic/endoplasmic reticulum calcium ATPase 1
UniProt Protein Name
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
UniProt Gene Name
ATP2A1
UniProt Synonym Gene Names
SERCA1; SR Ca(2+)-ATPase 1

NCBI Description

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Oct 2013]

Uniprot Description

Key regulator of striated muscle performance by acting as the major Ca2+ ATPase responsible for the reuptake of cytosolic Ca2+ into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.

Research Articles on ATP2A1

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Product Notes

The ATP2A1 atp2a1 (Catalog #AAA853289) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The ATP2A1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATP2A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the ATP2A1 atp2a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP2A1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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