Rabbit FXYD2 Polyclonal Antibody | anti-FXYD2 antibody

FXYD2 Polyclonal Antibody

Cat. Num. AAA3006493

• Gene Names: FXYD2; HOMG2; ATP1G1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: FXYD2; Polyclonal Antibody; FXYD2 Polyclonal Antibody; Sodium/potassium-transporting ATPase subunit gamma; Na(+)/K(+) ATPase subunit gamma; FXYD domain-containing ion transport; regulator 2; Sodium pump gamma chai; ATP1C; ATP1G1; anti-FXYD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FXYD2 polyclonal antibody detects endogenous levels of FXYD2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 64

• Applicable Applications for anti-FXYD2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human FXYD2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of FXYD2 polyclonal antibody at 1:500 dilutionLane1:HepG2 whole cell lysateLane2:Mouse lung tissue lysateLane3:Rat brain tissue lysate)

Related Product Information for anti-FXYD2 antibody

The human FXYD2 (pronounced fix-id) gene maps to chromosome 11q23 and encodes the gamma subunit of Na/K-ATPase (NKA). FXYD2 subunit modulates NKA activity by inducing ion channel activity. The mammalian FXYD family maintains Na+ and K+ gradients between the intracellular and extracellular milieus of cells in processes such as renal Na+-reabsorption, muscle contraction and neuronal excitability. FXYDs are single-span membrane proteins that share a 35 amino acid signature domain, beginning with the sequence PFXYD and containing 7 invariant and 6 conserved amino acids. Other members of the FXYD family include FXYD1 (PLM, phospholemman), FXYD3 (Mat8, mammary tumor protein 8), FXYD4 (CHIF) and FXYD5 (RIC).

NCBI and Uniprot Product Information

• NCBI GI #: 11125764
• NCBI GeneID: 486
• NCBI Accession #: NP_067614.1
• NCBI GenBank Nucleotide #: NP_067614.1
• UniProt Accession #: P54710
• Molecular Weight: ~ 7kDa
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit gamma isoform 2
• NCBI Official Synonym Full Names: FXYD domain containing ion transport regulator 2
• NCBI Official Symbol: FXYD2
• NCBI Official Synonym Symbols: HOMG2; ATP1G1
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit gamma
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit gamma
• Protein Family: Sodium/potassium-transporting ATPase
• UniProt Gene Name: FXYD2
• UniProt Synonym Gene Names: ATP1C; ATP1G1; Na(+)/K(+) ATPase subunit gamma
• UniProt Entry Name: ATNG_HUMAN

NCBI Description

This gene encodes a member of the FXYD family of transmembrane proteins. This particular protein encodes the sodium/potassium-transporting ATPase subunit gamma. Mutations in this gene have been associated with Renal Hypomagnesemia-2. Alternatively spliced transcript variants have been described. Read-through transcripts have been observed between this locus and the upstream FXYD domain-containing ion transport regulator 6 (FXYD6, GeneID 53826) locus.[provided by RefSeq, Feb 2011]

Uniprot Description

FXYD2: May be involved in forming the receptor site for cardiac glycoside binding or may modulate the transport function of the sodium ATPase. Defects in FXYD2 are the cause of hypomagnesemia type 2 (HOMG2); also known as dominant renal hypomagnesemia or hypomagnesemia with hypocalciuria. HOMG2 is a disorder due to primary renal wasting of magnesium. Plasma levels of other electrolytes are normal. The only abnormality found, in addition to low magnesium levels, is lowered renal excretion of calcium resulting in hypocalciuria. Belongs to the FXYD family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: intracellular membrane-bound organelle; basolateral plasma membrane; plasma membrane; sodium:potassium-exchanging ATPase complex

Molecular Function: transporter activity; ion channel activity; sodium:potassium-exchanging ATPase activity

Biological Process: transport; regulation of cell growth; transmembrane transport; potassium ion transport; regulation of cell proliferation

Disease: Hypomagnesemia 2, Renal