ERCC4 sirna

ERCC4 siRNA (Mouse)

Cat. Num. AAA8227578

• Gene Names: Ercc4; Xpf; AI606920
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ERCC4; ERCC4 siRNA (Mouse); DNA repair endonuclease XPF; DNA excision repair protein ERCC-4; ERCC4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ERCC4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 917

• Applicable Applications for ERCC4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ERCC4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ERCC4 sirna

siRNA to inhibit ERCC4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 241666402
• NCBI GeneID: 50505
• NCBI Accession #: NP_056584.2
• NCBI GenBank Nucleotide #: NM_015769.2
• UniProt Accession #: Q9QZD4; O54810; Q8R0I3
• Molecular Weight: 103,690 Da
• NCBI Official Full Name: DNA repair endonuclease XPF
• NCBI Official Synonym Full Names: excision repair cross-complementing rodent repair deficiency, complementation group 4
• NCBI Official Symbol: Ercc4
• NCBI Official Synonym Symbols: Xpf; AI606920
• NCBI Protein Information: DNA repair endonuclease XPF
• UniProt Protein Name: DNA repair endonuclease XPF
• Protein Family: ERCC4 domain-containing protein
• UniProt Gene Name: Ercc4
• UniProt Entry Name: XPF_MOUSE

Uniprot Description

ERCC4: Structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link. Defects in ERCC4 are the cause of xeroderma pigmentosum complementation group F (XP-F); also known as xeroderma pigmentosum VI (XP6). XP-F is an autosomal recessive disease characterized by hypersensitivity of the skin to sunlight followed by high incidence of skin cancer and frequent neurologic abnormalities. Defects in ERCC4 are a cause of XFE progeroid syndrome (XFEPS). This syndrome is illustrated by one patient who presented with dwarfism, cachexia and microcephaly. Belongs to the XPF family.

Protein type: Deoxyribonuclease; EC 3.1.-.-; DNA repair, damage

Cellular Component: transcription factor TFIID complex; nucleotide-excision repair complex; chromosome, telomeric region; nuclear chromosome, telomeric region; nucleus

Molecular Function: protein C-terminus binding; structure-specific DNA binding; nuclease activity; single-stranded DNA specific endodeoxyribonuclease activity; hydrolase activity; protein N-terminus binding; T/G mismatch-specific endonuclease activity; endodeoxyribonuclease activity; phosphoric ester hydrolase activity; protein binding; DNA binding; nucleic acid binding; endonuclease activity; damaged DNA binding; single-stranded DNA binding

Biological Process: nucleotide-excision repair, DNA incision; DNA repair; DNA catabolic process, endonucleolytic; double-strand break repair via homologous recombination; negative regulation of telomere maintenance; nucleotide-excision repair, DNA incision, 3'-to lesion; UV protection; nucleotide-excision repair; resolution of meiotic joint molecules as recombinants; response to DNA damage stimulus; nucleotide-excision repair, DNA incision, 5'-to lesion; DNA metabolic process; telomere maintenance; response to UV