Rabbit anti-Human TRF Polyclonal Antibody | anti-TRF antibody

TRF, CT (Telomeric Repeat Binding Factor 1, TTAGGG Repeat-binding Factor 1, NIMA-interacting Protein 2, Telomeric Protein Pin2/TRF1, TERF1, PIN2, TRBF1, TRF, TRF1) (Biotin)

Cat. Num. AAA6502285

• Gene Names: TERF1; TRF; PIN2; TRF1; TRBF1; t-TRF1; hTRF1-AS
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: TRF; Polyclonal Antibody; CT (Telomeric Repeat Binding Factor 1; TTAGGG Repeat-binding Factor 1; NIMA-interacting Protein 2; Telomeric Protein Pin2/TRF1; TERF1; PIN2; TRBF1; TRF1) (Biotin); anti-TRF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TERF1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TRF antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human TERF1 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-TERF1(C-term) Pab in Jurkat cell line lysates (35ug/lane). TERF1(C-term)(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-TRF antibody

Antibodies; Binding Proteins

References

1. Kim, M.K., J. Biol. Chem. 283(20), 14144-14152 (2008). 2. Etheridge, K.T., J. Biol. Chem. 283(11), 6935-6941 (2008). 3. Muramatsu, Y., Exp. Cell Res. 314(5), 1115-1124 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 189409142
• NCBI GeneID: 7013
• NCBI Accession #: NP_059523
• NCBI GenBank Nucleotide #: NM_017489.3
• UniProt Accession #: P54274
• Molecular Weight: 48kDa
• NCBI Official Full Name: telomeric repeat-binding factor 1 isoform 1
• NCBI Official Synonym Full Names: telomeric repeat binding factor 1
• NCBI Official Symbol: TERF1
• NCBI Official Synonym Symbols: TRF; PIN2; TRF1; TRBF1; t-TRF1; hTRF1-AS
• NCBI Protein Information: telomeric repeat-binding factor 1
• UniProt Protein Name: Telomeric repeat-binding factor 1
• Protein Family: TBP-related factor
• UniProt Gene Name: TERF1
• UniProt Synonym Gene Names: PIN2; TRBF1; TRF; TRF1
• UniProt Entry Name: TERF1_HUMAN

NCBI Description

This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq, Jul 2008]

Uniprot Description

TRF1: a telomeric repeat binding factor. Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Involved in the regulation of the mitotic spindle. Expression is tightly regulated during the cell cycle; levels are low in G1 and S phase and increase during G2 phase and mitosis. Two splice variant isoforms have been described.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: nucleoplasm; chromosome, telomeric region; nuclear telomere cap complex; nuclear chromosome, telomeric region; cytoplasm; nucleolus; spindle; nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; microtubule binding; telomeric DNA binding; double-stranded telomeric DNA binding; chromatin binding; DNA bending activity

Biological Process: response to drug; mitosis; positive regulation of mitosis; negative regulation of telomere maintenance via semi-conservative replication; positive regulation of apoptosis; positive regulation of microtubule polymerization; telomere maintenance via telomerase; positive regulation of mitotic cell cycle; negative regulation of telomerase activity; cell division; negative regulation of DNA replication; telomeric loop formation; mitotic cell cycle spindle assembly checkpoint; G2/M transition of mitotic cell cycle; age-dependent telomere shortening; telomere maintenance; negative regulation of telomere maintenance via telomerase; protein homooligomerization