ERBB3 sirna

ERBB3 siRNA (Mouse)

Cat. Num. AAA827326

• Gene Names: Erbb3; Her3; C76256; Erbb-3; Erbb3r
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ERBB3; ERBB3 siRNA (Mouse); Receptor tyrosine-protein kinase erbB-3; Glial growth factor receptor; Proto-oncogene-like protein c-ErbB-3; ERBB3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ERBB3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1339

• Applicable Applications for ERBB3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ERBB3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ERBB3 sirna

siRNA to inhibit ERBB3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 61098019
• NCBI GeneID: 13867
• NCBI Accession #: NP_034283.1
• NCBI GenBank Nucleotide #: NM_010153.1
• UniProt Accession #: Q61526; Q3KQR1; Q68J64; Q810U8; Q8K317
• Molecular Weight: 147,613 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-3
• NCBI Official Synonym Full Names: v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
• NCBI Official Symbol: Erbb3
• NCBI Official Synonym Symbols: Her3; C76256; Erbb-3; Erbb3r
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-3
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-3
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: Erbb3
• UniProt Entry Name: ERBB3_MOUSE

Uniprot Description

HER3: a receptor tyrosine kinase of the EGFR family. Binds and is activated by neuregulins and NTAK. Can form homodimers or ErbB-2/ErbB-3 heterodimers. Kinase domain lacks activity but heterodimerizes with other EGFRs to transduce growth signals. May be required for HER2 activity. Elevated expression in breast and other tumors is indicative of poor outcome. A secreted form is expressed in metastatic prostate cancer Two alternatively spliced isoforms have been described.

Protein type: EC 2.7.10.1; Kinase, protein; Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; Oncoprotein; TK group; EGFR family

Cellular Component: extracellular space; postsynaptic membrane; membrane; basolateral plasma membrane; apical plasma membrane; integral to membrane; nucleus; lateral plasma membrane; receptor complex

Molecular Function: identical protein binding; nucleotide binding; protein tyrosine kinase activator activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; protein kinase activity; transferase activity; protein binding; protein heterodimerization activity; growth factor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide 3-kinase cascade; cranial nerve development; negative regulation of cell adhesion; signal transduction; protein amino acid phosphorylation; regulation of cell proliferation; peripheral nervous system development; negative regulation of signal transduction; Schwann cell differentiation; positive regulation of glucose import; neuron apoptosis; negative regulation of secretion; negative regulation of neuron apoptosis; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway