HER3 / ErbB3 recombinant protein

Recombinant Mouse HER3 / ErbB3 Protein (His tag)

Cat. Num. AAA2546694

• Gene Names: Erbb3; Her3; C76256; Erbb-3; Erbb3r
• Purity: > 93 % as determined by SDS-PAGE
• Synonyms: HER3 / ErbB3; Recombinant Mouse HER3 / ErbB3 Protein (His tag); C76256; Erbb-3; Erbb3r; Her3; MGC117742; HER3 / ErbB3 recombinant protein

• Host: Human Cells
• Purity/Purification: > 93 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 1339

• Application Notes: The secreted recombinant mouse ERBB3 comprises 633 amino acids and has a calculated molecular mass of 70 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 95 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ser 20
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for HER3 / ErbB3 recombinant protein

Background: ErbB3, also known as Her3 (human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

Description: A DNA sequence encoding the mouse ERBB3 (Q61526) extracellular domain (Met 1-His 641) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 61098019
• NCBI GeneID: 13867
• NCBI Accession #: NP_034283.1
• NCBI GenBank Nucleotide #: NM_010153.1
• UniProt Accession #: Q61526; Q3KQR1; Q68J64; Q810U8; Q8K317
• Molecular Weight: 147,613 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-3
• NCBI Official Synonym Full Names: erb-b2 receptor tyrosine kinase 3
• NCBI Official Symbol: Erbb3
• NCBI Official Synonym Symbols: Her3; C76256; Erbb-3; Erbb3r
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-3
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-3
• UniProt Gene Name: Erbb3

Uniprot Description

HER3: a receptor tyrosine kinase of the EGFR family. Binds and is activated by neuregulins and NTAK. Can form homodimers or ErbB-2/ErbB-3 heterodimers. Kinase domain lacks activity but heterodimerizes with other EGFRs to transduce growth signals. May be required for HER2 activity. Elevated expression in breast and other tumors is indicative of poor outcome. A secreted form is expressed in metastatic prostate cancer Two alternatively spliced isoforms have been described.

Protein type: EC 2.7.10.1; EGFR family; Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 10 D3|10 77.1 cM

Cellular Component: apical plasma membrane; basolateral plasma membrane; extracellular space; lateral plasma membrane; nucleus; postsynaptic membrane; receptor complex

Molecular Function: growth factor binding; protein binding; protein heterodimerization activity; protein tyrosine kinase activator activity; protein-tyrosine kinase activity

Biological Process: cranial nerve development; epidermal growth factor receptor signaling pathway; negative regulation of cell adhesion; negative regulation of neuron apoptosis; negative regulation of secretion; negative regulation of signal transduction; neuron apoptosis; peripheral nervous system development; phosphoinositide 3-kinase cascade; positive regulation of cardiac muscle development; positive regulation of glucose import; regulation of cell proliferation; Schwann cell differentiation; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway