EPHA7 sirna

EPHA7 siRNA (Mouse)

Cat. Num. AAA8204724

• Gene Names: Epha7; Ebk; Ehk3; Mdk1; Cek11; Hek11
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EPHA7; EPHA7 siRNA (Mouse); EBK; EHK3; MDK1; Ephrin type-A receptor 7; Developmental kinase 1; mDK-1; EPH homology kinase 3; EHK-3; Embryonic brain kinase; EPHA7 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EPHA7 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 994

• Applicable Applications for EPHA7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EPHA7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EPHA7 sirna

siRNA to inhibit EPHA7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 594190862
• NCBI GeneID: 13841
• NCBI Accession #: NP_001277363.1
• NCBI GenBank Nucleotide #: NM_001290434.1
• UniProt Accession #: Q61772; Q61505; Q61773; Q61774; Q8BSU8
• Molecular Weight: 70,300 Da
• NCBI Official Full Name: ephrin type-A receptor 7 isoform 3
• NCBI Official Synonym Full Names: Eph receptor A7
• NCBI Official Symbol: Epha7
• NCBI Official Synonym Symbols: Ebk; Ehk3; Mdk1; Cek11; Hek11
• NCBI Protein Information: ephrin type-A receptor 7
• UniProt Protein Name: Ephrin type-A receptor 7
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: Epha7
• UniProt Synonym Gene Names: Ebk; Ehk3; Mdk1; mDK-1; EHK-3; EBK
• UniProt Entry Name: EPHA7_MOUSE

Uniprot Description

EphA7: a widely-expressed receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A2, -A3, -A4 and -A5. Contains 1 sterile alpha motif (SAM) and two fibronectin type III domains.

Protein type: Membrane protein, integral; Protein kinase, TK; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; Eph family

Cellular Component: postsynaptic membrane; membrane; cell soma; integral to plasma membrane; dendrite; plasma membrane; integral to membrane; neuromuscular junction

Molecular Function: ephrin receptor binding; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; protein kinase activity; transferase activity; protein binding; chemorepellent activity; GPI-linked ephrin receptor activity; protein-tyrosine kinase activity; ephrin receptor activity; transferase activity, transferring phosphorus-containing groups; kinase activity; axon guidance receptor activity; ATP binding

Biological Process: nervous system development; peptidyl-tyrosine phosphorylation; branching morphogenesis of a nerve; apoptosis; positive regulation of apoptosis; multicellular organismal development; regulation of protein amino acid autophosphorylation; negative chemotaxis; regulation of caspase activity; signal transduction; protein amino acid phosphorylation; regulation of cell-cell adhesion; positive regulation of neuron apoptosis; ephrin receptor signaling pathway; brain development; phosphorylation; regulation of peptidyl-tyrosine phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; retinal ganglion cell axon guidance