EphA7 / EHK-3 recombinant protein

Recombinant Mouse EphA7 / EHK-3 Protein (His tag)

Cat. Num. AAA2546895

• Gene Names: Epha7; Ebk; Ehk3; Mdk1; Cek11; Hek11
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: EphA7 / EHK-3; Recombinant Mouse EphA7 / EHK-3 Protein (His tag); RP23-33D17.1; Cek11; Ebk; Ehk3; Hek11; Mdk1; EphA7 / EHK-3 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 994

• Application Notes: The secreted recombinant mouse EPHA7 consists of 537 amino acids and has a predicted molecular mass of 60.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEPHA7 is approximately 70 kDa due to glycosylation.
• Predicted N Terminal: Lys 30
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for EphA7 / EHK-3 recombinant protein

Background: Ephrin type -A receptor 7, also known as EphA7, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Eph receptor-mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. Down-regulation of EphA7 secondary to hypermethylation has been reported in colorectal cancer. The expression of EphA7 was reduced in all tested gastric cancer cell lines; however, there is marked variability in expression among gastric carcinoma specimens. EphA7 may have roles in the pathogenesis and development of gastric carcinomas.

Description: A DNA sequence encoding the mouse EPHA7 isoform 1 (Q61772-1) extracellular domain (Met 1-Ile 556) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 594190862
• NCBI GeneID: 13841
• NCBI Accession #: NP_001277363.1
• NCBI GenBank Nucleotide #: NM_001290434.1
• UniProt Accession #: Q61772; Q61505; Q61773; Q61774; Q8BSU8
• Molecular Weight: 70,300 Da
• NCBI Official Full Name: ephrin type-A receptor 7 isoform 3
• NCBI Official Synonym Full Names: Eph receptor A7
• NCBI Official Symbol: Epha7
• NCBI Official Synonym Symbols: Ebk; Ehk3; Mdk1; Cek11; Hek11
• NCBI Protein Information: ephrin type-A receptor 7
• UniProt Protein Name: Ephrin type-A receptor 7
• UniProt Gene Name: Epha7
• UniProt Synonym Gene Names: Ebk; Ehk3; Mdk1; mDK-1; EHK-3; EBK

Uniprot Description

EphA7: a widely-expressed receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A2, -A3, -A4 and -A5. Contains 1 sterile alpha motif (SAM) and two fibronectin type III domains.

Protein type: EC 2.7.10.1; Eph family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 4 A4|4 12.42 cM

Cellular Component: cell soma; dendrite; integral to plasma membrane; neuromuscular junction; postsynaptic membrane

Molecular Function: axon guidance receptor activity; chemorepellent activity; ephrin receptor binding; GPI-linked ephrin receptor activity; protein binding; protein-tyrosine kinase activity

Biological Process: brain development; branching morphogenesis of a nerve; ephrin receptor signaling pathway; negative regulation of collateral sprouting; negative regulation of synaptogenesis; phosphorylation; positive regulation of apoptosis; positive regulation of neuron apoptosis; positive regulation of protein amino acid phosphorylation; regulation of caspase activity; regulation of cell-cell adhesion; regulation of peptidyl-tyrosine phosphorylation; regulation of protein amino acid autophosphorylation; retinal ganglion cell axon guidance