EphA7 active protein

EphA7 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122849

• Gene Names: Epha7; Ebk; Ehk3; Mdk1; Cek11; Hek11
• Purity: >97% as determined by SDS-PAGE
• Synonyms: EphA7; EphA7 Protein; Mouse; Recombinant (His Tag); Mouse EphA7/EHK-3 Protein (His Tag); Cek11 Protein; Ebk Protein; Ehk3 Protein; Hek11 Protein; Mdk1 Protein; RP23-33D17.1 Protein; Eph receptor A7; EphA7 active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ile556

• Species: Mouse
• Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse EphA7 at 2ug/ml (100uL/well) can bind mouse EphrinA4 with a linear range of 0.08-10ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Lys 30
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse EPHA7 isoform 1 (Q61772-1) extracellular domain (Met 1-Ile 556) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for EphA7 active protein

Background: Ephrin type-A receptor 7, also known as EphA7, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Eph receptor-mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. Down-regulation of EphA7 secondary to hypermethylation has been reported in colorectal cancer. The expression of EphA7 was reduced in all tested gastric cancer cell lines; however, there is marked variability in expression among gastric carcinoma specimens. EphA7 may have roles in the pathogenesis and development of gastric carcinomas.

Product Categories/Family for EphA7 active protein

Actin Dynamics Signaling Pathway

References

Rashid T, et al. (2005) Opposing gradients of ephrin-As and EphA7 in the superior colliculus are essential for topographic mapping in the mammalian visual system. Neuron. 47(1): 57-69.Wang J, et al. (2007) Differential expression of EphA7 receptor tyrosine kinase in gastric carcinoma. Hum Pathol. 38(11): 1649-56.Rogers JH, et al. (1999) Distribution of the receptor EphA7 and its ligands in development of the mouse nervous system. Brain Res Mol Brain Res. 74(1-2): 225-30.

NCBI and Uniprot Product Information

• NCBI GI #: 171541811
• NCBI GeneID: 13841
• NCBI Accession #: NP_034271.3
• NCBI GenBank Nucleotide #: NM_010141.3
• UniProt Accession #: Q61772; Q61505; Q61773; Q61774; Q8BSU8
• Molecular Weight: 111,860 Da
• NCBI Official Full Name: ephrin type-A receptor 7 isoform 1
• NCBI Official Synonym Full Names: Eph receptor A7
• NCBI Official Symbol: Epha7
• NCBI Official Synonym Symbols: Ebk; Ehk3; Mdk1; Cek11; Hek11
• NCBI Protein Information: ephrin type-A receptor 7; EHK-3; mDK-1; EPH homology kinase 3; developmental kinase 1; embryonic brain kinase
• UniProt Protein Name: Ephrin type-A receptor 7
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: Epha7
• UniProt Synonym Gene Names: Ebk; Ehk3; Mdk1; mDK-1; EHK-3; EBK
• UniProt Entry Name: EPHA7_MOUSE

Uniprot Description

Function: Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 AND MAPK3 which are phosphorylated upon activation of EPHA7. Isoform 4 which lacks the kinase domain may regulate isoform 1 adhesive properties. Ref.8 Ref.9 Ref.10 Ref.11

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure: Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses

By similarity. Interacts (via PDZ-binding motif) with GRIP1 and PICK1 (via PDZ domain). Ref.7

Subcellular location: Cell membrane

Probable; Single-pass type I membrane protein

Probable Ref.8.

Tissue specificity: Widely expressed in embryo. In adult, expression restricted to hippocampus, testis and spleen.

Developmental stage: During visual system development, expressed in an anterior to posterior decreasing gradient stretching through the entire midbrain. This gradient has the reverse orientation to the one defined by the expression of ephrins. Isoform 4 and isoform 5 are expressed at the edges of the embryonic cranial neural fold. Ref.8 Ref.10

Post-translational modification: Phosphorylated. Isoform 4 inhibits isoform 1 phosphorylation and may regulate its function in adhesion. Ref.8

Disruption phenotype: Mice are viable, fertile and show no gross morphological or behavioral defects. However, topographic targeting errors of nasal and temporal retinal axons appear during development of the retinocollicular projections in the visual system. 10 percent of the embryos also display exencephalic overgrowth of forebrain tissues which might be the result of reduced apoptosis. Ref.9 Ref.10

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.Contains 1 Eph LBD (Eph ligand-binding) domain.Contains 2 fibronectin type-III domains.Contains 1 protein kinase domain.Contains 1 SAM (sterile alpha motif) domain.