DBP sirna

DBP siRNA (Mouse)

Cat. Num. AAA827122

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DBP; DBP siRNA (Mouse); D site-binding protein; Albumin D box-binding protein; Albumin D-element-binding protein; DBP sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DBP siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 325

• Applicable Applications for DBP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DBP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DBP sirna

siRNA to inhibit DBP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 170650717
• NCBI GeneID: 13170
• NCBI Accession #: NP_058670.2
• NCBI GenBank Nucleotide #: NM_016974.3
• UniProt Accession #: Q60925; Q8VCX3
• Molecular Weight: 34,380 Da
• NCBI Official Full Name: D site-binding protein
• NCBI Official Synonym Full Names: D site albumin promoter binding protein
• NCBI Official Symbol: Dbp
• NCBI Protein Information: D site-binding protein
• UniProt Protein Name: D site-binding protein
• Protein Family: D site-binding protein
• UniProt Gene Name: Dbp
• UniProt Entry Name: DBP_MOUSE

NCBI Description

The protein encoded by this gene is a member of the Par bZIP transcription factor family and binds to specific sequences in the promoters of several genes, such as albumin, Cyp2a4, and Cyp2a5. The encoded protein can bind DNA as a homo- or heterodimer and is involved in the regulation of some circadian rhythym genes. [provided by RefSeq, Feb 2014]

Uniprot Description

DBP: This transcriptional activator recognizes and binds to the sequence 5'-RTTAYGTAAY-3' found in the promoter of genes such as albumin, CYP2A4 and CYP2A5. It is not essential for circadian rhythm generation, but modulates important clock output genes. May be a direct target for regulation by the circadian pacemaker component clock. May affect circadian period and sleep regulation. Belongs to the bZIP family. PAR subfamily.

Protein type: DNA-binding; Transcription factor

Cellular Component: nucleus

Molecular Function: DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; regulation of transcription, DNA-dependent; rhythmic process; positive regulation of transcription from RNA polymerase II promoter