Rabbit Hemagglutinin Tag Polyclonal Antibody

Hemagglutinin Tag (HA Tag, CYPYDVPDYAYPYDVPDYA) (Azide free) (HRP)

Cat. Num. AAA6306080

• Applications: ELISA, Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Hemagglutinin Tag; Polyclonal Antibody; Hemagglutinin Tag (HA Tag; CYPYDVPDYAYPYDVPDYA) (Azide free) (HRP); Tag from influenza hemagglutinin protein; anti-Hemagglutinin Tag antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Hemagglutinin Tag
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Hemagglutinin Tag antibody: ELISA (EIA), Western Blot (WB), Chromatin Immunoprecipitation (ChIP)
• Application Notes: ELISA: 1:5,000; Applications are based on unconjugated antibody.
• Immunogen: KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-HA tag pabin HA-tagged recombinant protein bacterial lysate.)

Testing Data

(GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko:Journal of Cell Science 120, 3188-3199 (2007)))

Chromatin Immunoprecipitation (ChIP)

(ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 provided a control(Provided by Shucai Wang, Su-Hwan Kwak:Development 134, 3873-3882 (2007)))

Related Product Information for anti-Hemagglutinin Tag antibody

Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. Our anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.

Product Categories/Family for anti-Hemagglutinin Tag antibody

Antibodies; Epitope Tag; HA

References

Marco. et.al., J Biol Chem. (2009). February 6; 284(6): 3577 585. Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. Sells MA and Chernoff J. (1995) Gene, 152:187-9.