Rabbit anti-Human, Mouse PON2 Polyclonal Antibody | anti-PON2 antibody

PON2, ID (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (PE)

Cat. Num. AAA6335894

• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PON2; Polyclonal Antibody; ID (PON2; Serum paraoxonase/arylesterase 2; Aromatic esterase 2; Serum aryldialkylphosphatase 2) (PE); Serum aryldialkylphosphatase 2; anti-PON2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PON2 antibody: Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PON2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-110 amino acids from the Central region of human PON2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PON2 Antibody (Center) western blot analysis in HepG2 cell line lysates (15ug/lane).This demonstrates the PON2 antibody detected PON2 protein (arrow).)

Western Blot (WB)

(PON2 Antibody (Center) western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the PON2 antibody detected PON2 protein (arrow).)

Immunohistochemistry (IHC)

(PON2 antibody(Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PON2 antibody(Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of PON2 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-PON2 antibody

PON2 is a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes.

Product Categories/Family for anti-PON2 antibody

Antibodies; Enzymes; Phosphatase

References

Sanghera, D.K., et al. Am. J. Hum. Genet. 62(1):36-44(1998). Hegele, R.A., et al. J. Clin. Endocrinol. Metab. 82(10):3373-3377(1997). Primo-Parmo, S.L., et al. Genomics 33(3):498-507(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 66529294
• NCBI GeneID: 5445
• NCBI Accession #: NP_000296.2
• NCBI GenBank Nucleotide #: NM_000305.2
• UniProt Accession #: Q15165; O15114; O15115; O75856; Q5FBX7; Q86YL0; A4D1H7; B2RCP9; B4DJD5
• Molecular Weight: 43.5 kDa
• NCBI Official Full Name: serum paraoxonase/arylesterase 2 isoform 1
• NCBI Official Synonym Full Names: paraoxonase 2
• NCBI Official Symbol: PON2
• NCBI Protein Information: serum paraoxonase/arylesterase 2; A-esterase 2; PON 2; aromatic esterase 2; paraoxonase nirs; serum aryldialkylphosphatase 2
• UniProt Protein Name: Serum paraoxonase/arylesterase 2
• Protein Family: Serum paraoxonase/arylesterase
• UniProt Gene Name: PON2
• UniProt Synonym Gene Names: PON 2; A-esterase 2
• UniProt Entry Name: PON2_HUMAN

NCBI Description

This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

PON2: Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Belongs to the paraoxonase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.81; Hydrolase; EC 3.1.1.2; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: mitochondrion; lysosome; plasma membrane; extracellular region; nucleus

Molecular Function: identical protein binding; arylesterase activity; metal ion binding

Biological Process: response to oxidative stress; aromatic compound catabolic process