Western Blot (WB)
(Western blot analysis of multiple Cell line lysates showing detection of AKT2 protein using Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)
Rabbit Akt2 Polyclonal Antibody | anti-AKT2 antibody
Akt2 (PKB beta) Antibody: Alkaline Phosphatase
Gene Names
AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus.
Applications
Western Blot, ELISA
Synonyms
Akt2; Polyclonal Antibody; Akt2 (PKB beta) Antibody: Alkaline Phosphatase; AKT2; PKB; PKB beta; PRKBB; protein kinase Akt2; RAC BETA; anti-AKT2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus.
Clonality
Polyclonal
Form/Format
Affinity Purified
Concentration
0.25mg/mL (varies by lot)
Sequence Length
481
Applicable Applications for anti-AKT2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
1:3000 dilution was sufficient for detection of PKBbeta in 20 ug of HeLa cell lysate by ECL immunoblot analysis.
Conjugate
Alkaline Phosphatase
Storage Buffer
TBS, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Western Blot (WB)
(Western blot analysis of multiple Cell line lysates showing detection of AKT2 protein using Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)
Western Blot (WB)
(Western blot analysis of multiple Cell line lysates showing detection of AKT2 protein using Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)
Related Product Information for anti-AKT2 antibody
Background Info: Detects a 65kDa protein, corresponding to the molecular mass of Akt2 PKBbeta on SDS PAGE immunoblots. Does not react with PKBalpha (Akt1) or PKBgamma (Akt3).
Scientific Background: Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).
Scientific Background: Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).
Product Categories/Family for anti-AKT2 antibody
References
1. Downward J (1998). Curr Opin Cell Biol. 10(2): 262-7. 2. Brazil DP, and Hemmings BA (2001). Biochem Sci. 26(11):657-64. 3. Nicholson KM, Anderson NG (2002). Cell Signal. 14(5): 381-95. 4. Hardie, G., Hanks, S. (1995). The Protein Kinase Facts Book, Protein-Serine Kinases Academic Press Limited, San Diego, CA 418pps.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,769 Da
NCBI Official Full Name
RAC-beta serine/threonine-protein kinase isoform 1
NCBI Official Synonym Full Names
v-akt murine thymoma viral oncogene homolog 2
NCBI Official Symbol
AKT2
NCBI Official Synonym Symbols
PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
NCBI Protein Information
RAC-beta serine/threonine-protein kinase; PKB beta; RAC-PK-beta; protein kinase Akt-2; protein kinase B beta; rac protein kinase beta; murine thymoma viral (v-akt) homolog-2
UniProt Protein Name
RAC-beta serine/threonine-protein kinase
Protein Family
UniProt Gene Name
AKT2
UniProt Synonym Gene Names
PKB beta
UniProt Entry Name
AKT2_HUMAN
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Product Notes
The AKT2 akt2 (Catalog #AAA805060) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Akt2 (PKB beta) Antibody: Alkaline Phosphatase reacts with Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus. and may cross-react with other species as described in the data sheet. AAA Biotech's Akt2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). 1:3000 dilution was sufficient for detection of PKBbeta in 20 ug of HeLa cell lysate by ECL immunoblot analysis. Researchers should empirically determine the suitability of the AKT2 akt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Akt2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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