CLP1 sirna

CLP1 siRNA (Mouse)

Cat. Num. AAA8205537

• Gene Names: Clp1; Heab; AI462438
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CLP1; CLP1 siRNA (Mouse); Polyribonucleotide 5'-hydroxyl-kinase Clp1; Polyadenylation factor Clp1; Polynucleotide kinase Clp1; Pre-mRNA cleavage complex II protein Clp1; CLP1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CLP1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 425

• Applicable Applications for CLP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CLP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CLP1 sirna

siRNA to inhibit CLP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19527056
• NCBI GeneID: 98985
• NCBI Accession #: NP_598601.1
• NCBI GenBank Nucleotide #: NM_133840.2
• UniProt Accession #: Q99LI9; Q3TEC7; Q3ULZ9
• Molecular Weight: 47,738 Da
• NCBI Official Full Name: polyribonucleotide 5'-hydroxyl-kinase Clp1
• NCBI Official Synonym Full Names: CLP1, cleavage and polyadenylation factor I subunit
• NCBI Official Symbol: Clp1
• NCBI Official Synonym Symbols: Heab; AI462438
• NCBI Protein Information: polyribonucleotide 5'-hydroxyl-kinase Clp1
• UniProt Protein Name: Polyribonucleotide 5'-hydroxyl-kinase Clp1
• Protein Family: Tyrosine-protein phosphatase
• UniProt Gene Name: Clp1
• UniProt Entry Name: CLP1_MOUSE

Uniprot Description

HEAB: Polynucleotide kinase that can phosphorylate the 5'- hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA- induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing. Belongs to the Clp1 family. Clp1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding; EC 2.7.1.78

Cellular Component: nucleoplasm; cytoplasm; nucleus; mRNA cleavage factor complex; tRNA-intron endonuclease complex

Molecular Function: transferase activity; polydeoxyribonucleotide kinase activity; ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; nucleotide binding; kinase activity; ATP-dependent polyribonucleotide 5'-hydroxyl-kinase activity; polynucleotide kinase activity; ATP binding

Biological Process: cerebellar cortex development; tRNA splicing; RNA interference, siRNA loading onto RISC; tRNA processing; RNA interference, targeting of mRNA for destruction; mRNA polyadenylation; mRNA 3'-end processing; mRNA processing; phosphorylation; mRNA cleavage