Western Blot (WB)
(GPR109B Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the GPR109B antibody detected the GPR109B protein (arrow).)
Rabbit anti-Human GPR109B Polyclonal Antibody | anti-HCAR3 antibody
GPR109B, NT (HCAR3, GPR109B, HCA3, HM74B, NIACR2, Hydroxycarboxylic acid receptor 3, Nicotinic acid receptor 2, G-protein coupled receptor 109B, G-protein coupled receptor HM74, G-protein coupled receptor HM74B, Niacin receptor 2)
Gene Names
HCAR3; HCA3; HM74; PUMAG; Puma-g; GPR109B
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
GPR109B; Polyclonal Antibody; NT (HCAR3; HCA3; HM74B; NIACR2; Hydroxycarboxylic acid receptor 3; Nicotinic acid receptor 2; G-protein coupled receptor 109B; G-protein coupled receptor HM74; G-protein coupled receptor HM74B; Niacin receptor 2); Anti -GPR109B; anti-HCAR3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-HCAR3 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
GPR109B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-99 amino acids from the N-terminal region of human GPR109B.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(GPR109B Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the GPR109B antibody detected the GPR109B protein (arrow).)
Western Blot (WB)
(GPR109B Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the GPR109B antibody detected the GPR109B protein (arrow).)
Related Product Information for anti-HCAR3 antibody
Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteract prolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as a low affinity receptor for nicotinic acid. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet.
Product Categories/Family for anti-HCAR3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
44,478 Da
NCBI Official Full Name
G protein-coupled receptor 109B
NCBI Official Synonym Full Names
hydroxycarboxylic acid receptor 3
NCBI Official Symbol
HCAR3
NCBI Official Synonym Symbols
HCA3; HM74; PUMAG; Puma-g; GPR109B
NCBI Protein Information
hydroxycarboxylic acid receptor 3; niacin receptor 2; GTP-binding protein; nicotinic acid receptor 2; putative chemokine receptor; G protein-coupled receptor 109B; G-protein coupled receptor 109B; G-protein coupled receptor HM74; G-protein coupled receptor HM74B; hydroxy-carboxylic acid receptor 3
UniProt Protein Name
Hydroxycarboxylic acid receptor 3
Protein Family
UniProt Gene Name
HCAR3
UniProt Synonym Gene Names
GPR109B; HCA3; HM74B; NIACR2
UniProt Entry Name
HCAR3_HUMAN
Uniprot Description
Function: Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteract prolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as a low affinity receptor for nicotinic acid. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Ref.7 Ref.8
Subcellular location: Cell membrane; Multi-pass membrane protein.
Tissue specificity: Expression largely restricted to adipose tissue and spleen. Ref.7
Sequence similarities: Belongs to the G-protein coupled receptor 1 family.
Research Articles on HCAR3
Similar Products
Product Notes
The HCAR3 hcar3 (Catalog #AAA646420) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR109B, NT (HCAR3, GPR109B, HCA3, HM74B, NIACR2, Hydroxycarboxylic acid receptor 3, Nicotinic acid receptor 2, G-protein coupled receptor 109B, G-protein coupled receptor HM74, G-protein coupled receptor HM74B, Niacin receptor 2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GPR109B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the HCAR3 hcar3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR109B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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