Bovine Hyaluronidase-1 (HYAL1) ELISA Kit | HYAL1 elisa kit

Bovine Hyaluronidase-1 (HYAL1) ELISA Kit

Cat. Num. AAA7238849

• Gene Names: HYAL1; NAT6; LUCA1; HYAL-1
• Reactivity: Bovine
• Synonyms: Hyaluronidase-1 (HYAL1); Bovine Hyaluronidase-1 (HYAL1) ELISA Kit; HYAL1 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1ng/mL.
• Intended Uses: This HYAL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine HYAL1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HYAL1 elisa kit

Principle of the assay: HYAL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HYAL1 antibody and an HYAL1-HRP conjugate. The assay sample and buffer are incubated together with HYAL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HYAL1 concentration since HYAL1 from samples and HYAL1-HRP conjugate compete for the anti-HYAL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HYAL1 from the sample, fewer sites are left to bind HYAL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HYAL1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for HYAL1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 24497566
• NCBI GeneID: 3373
• NCBI Accession #: NP_695014.1
• NCBI GenBank Nucleotide #: NM_153282.2
• Molecular Weight: 37,439 Da
• NCBI Official Full Name: hyaluronidase-1 isoform 2
• NCBI Official Synonym Full Names: hyaluronoglucosaminidase 1
• NCBI Official Symbol: HYAL1
• NCBI Official Synonym Symbols: NAT6; LUCA1; HYAL-1
• NCBI Protein Information: hyaluronidase-1; luCa-1; plasma hyaluronidase; tumor suppressor LUCA-1; lung carcinoma protein 1
• UniProt Protein Name: Hyaluronidase-1
• UniProt Gene Name: HYAL1
• UniProt Synonym Gene Names: LUCA1; Hyal-1; LuCa-1
• UniProt Entry Name: HYAL1_HUMAN

NCBI Description

This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

HYAL1: May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth. Defects in HYAL1 are the cause of mucopolysaccharidosis type 9 (MPS9); also called hyaluronidase deficiency. MPS9 is a lysosomal storage disease characterized by high hyaluronan (HA) concentration in the serum. The clinical features are periarticular soft tissue masses, mild short stature and acetabular erosions, and absence of neurological or visceral involvement. Belongs to the glycosyl hydrolase 56 family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; EC 3.2.1.35; Glycan Metabolism - glycosaminoglycan degradation; Secreted; Hydrolase

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: lysosomal lumen; extracellular space; lysosome; cytoplasm; cytoplasmic vesicle

Molecular Function: viral receptor activity; hyaluronan synthase activity; transcription factor binding; hyalurononglucosaminidase activity

Biological Process: positive regulation of cell adhesion; glycosaminoglycan metabolic process; response to virus; pathogenesis; positive regulation of cell growth; hyaluronan catabolic process; response to antibiotic; chondroitin sulfate metabolic process; positive regulation of angiogenesis; response to reactive oxygen species; hyaluronan biosynthetic process; cartilage development; carbohydrate metabolic process; chondroitin sulfate catabolic process; negative regulation of cell growth; inflammatory response; positive regulation of growth; hyaluronan metabolic process; positive regulation of epithelial cell proliferation

Disease: Mucopolysaccharidosis, Type Ix