CIT sirna

CIT siRNA (Mouse)

Cat. Num. AAA8216094

• Gene Names: Cit; CRIK; Cit-k; CRIK-SK; C030025P15Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CIT; CIT siRNA (Mouse); CRIK; Citron Rho-interacting kinase; Rho-interacting. serine/threonine-protein kinase 21; CIT sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CIT siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2,085

• Applicable Applications for CIT sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CIT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CIT sirna

siRNA to inhibit CIT expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 568936836
• NCBI GeneID: 12704
• NCBI Accession #: XP_006530214.1
• NCBI GenBank Nucleotide #: XM_006530151.2
• UniProt Accession #: P49025; O88528; O88937; O88938; Q3UM99; Q8CIJ1
• Molecular Weight: 238,846 Da
• NCBI Official Full Name: citron Rho-interacting kinase isoform X18
• NCBI Official Synonym Full Names: citron
• NCBI Official Symbol: Cit
• NCBI Official Synonym Symbols: CRIK; Cit-k; CRIK-SK; C030025P15Rik
• NCBI Protein Information: citron Rho-interacting kinase
• UniProt Protein Name: Citron Rho-interacting kinase
• Protein Family: Citrate
• UniProt Gene Name: Cit
• UniProt Synonym Gene Names: Crik; CRIK
• UniProt Entry Name: CTRO_MOUSE

Uniprot Description

CRIK: an AGC kinase that is a RHO/RAC effector. Binds to the GTP-bound forms of RHO and RAC1. Required for postnatal hippocampal neurogenesis. Rho/CRIK suppresses differentiation and is associated with induction of KyoT1/2, a LIM domain protein gene implicated in integrin-mediated processes and Notch signaling. Plays an important role in the regulation of cytokinesis and the development of the central nervous system. Overexpressed in mantle-cell lymphoma (MCL), an aggressive type of B-cell lymphoma. Three alternatively spliced isoforms have been described.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; AGC group; DMPK family

Cellular Component: ruffle; Golgi cisterna; membrane; cell soma; cytoplasm; plasma membrane; vacuole; actin cytoskeleton

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; Rho GTPase binding; metal ion binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; SH3 domain binding; ATP binding; PDZ domain binding; protein kinase activity

Biological Process: positive regulation of cytokinesis; nervous system development; mitosis; mitotic sister chromatid segregation; multicellular organismal development; regulation of actin polymerization and/or depolymerization; cytokinesis; negative regulation of dendrite morphogenesis; cell cycle; protein amino acid phosphorylation; negative regulation of neuron differentiation; cell division; generation of neurons; dendrite development; spermatogenesis; cell differentiation; G2/M transition of mitotic cell cycle; phosphorylation; Golgi organization and biogenesis; mitotic metaphase/anaphase transition