Rabbit RWDD3 Polyclonal Antibody | anti-RWDD3 antibody

RWDD3 Antibody

Cat. Num. AAA150223

• Gene Names: RWDD3; RSUME
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: RWDD3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: RWDD3; Polyclonal Antibody; RWDD3 Antibody; RSUME; RWD domain-containing protein 3; RWD-containing sumoylation enhancer; RWD domain containing 3; anti-RWDD3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: RWDD3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 267

• Applicable Applications for anti-RWDD3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: RWDD3 antibody can be used for detection of RWDD3 by Western blot at 1 mug/mL.
• Conjugate: Unconjugated
• Immunogen: RWDD3 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human RWDD3.
• Buffer: RWDD3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: RWDD3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of RWDD3 in rat kidney tissue lysate with RWDD3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide. )

Related Product Information for anti-RWDD3 antibody

RWDD3 Antibody: RWDD3 (RSUME), a small RWD-containing protein, has a central role in sumoylation by enhancing SUMO conjugation in the regulatory network of immune-inflammatory signals. RWDD3 increases IκBeta sumoylation and stability. In addition, RWDD3 inhibits TNF-α-induced kappaB-LUC (Luciferase) reporter activity, showing the functional consequence of IκBeta increased stability. RSUME-enhanced sumoylation of IκBeta leads to the inhibition of NF-κBeta activity on two well-known inflammatory genes, IL-8 and cyclooxygenase-2 (Cox-2) and therefore may also favor anti-inflammatory pathways. Expression of RWDD3 was induced under hypoxic conditions and it has a potential role during vascularization. Both BMP-4 and RWDD3 may be interesting targets for inhibiting steps involved in pituitary tumorigenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 153252154
• NCBI GeneID: 25950
• NCBI Accession #: NP_056300
• NCBI GenBank Nucleotide #: NM_015485.4
• UniProt Accession #: Q9Y3V2; Q08AJ7; Q6FID3; Q9BX35; A6NP44; A8K9F0; C9J9L7; C9JI45
• Molecular Weight: 22,857 Da
• NCBI Official Full Name: RWD domain-containing protein 3 isoform a
• NCBI Official Synonym Full Names: RWD domain containing 3
• NCBI Official Symbol: RWDD3
• NCBI Official Synonym Symbols: RSUME
• NCBI Protein Information: RWD domain-containing protein 3; RWD-containing sumoylation enhancer; RWD domain-containing sumoylation enhancer
• UniProt Protein Name: RWD domain-containing protein 3
• Protein Family: RWD domain-containing protein
• UniProt Gene Name: RWDD3
• UniProt Synonym Gene Names: RSUME; RSUME
• UniProt Entry Name: RWDD3_HUMAN

Uniprot Description

RWDD3: Enhances the sumoylation of a number of proteins including HIF1A, PIAS and I-kappa-B, through direct interaction with UBC9. Has no effect on ubiquitination. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: SUMO conjugating system

Chromosomal Location of Human Ortholog: 1p21.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: positive regulation of protein sumoylation; inhibition of NF-kappaB transcription factor