CCR3 sirna

CCR3 siRNA (Mouse)

Cat. Num. AAA8201918

• Gene Names: Ccr3; CKR3; Cmkbr3; CC-CKR3; Cmkbr1l2
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CCR3; CCR3 siRNA (Mouse); CMKBR1L2; CMKBR3; Probable C-C chemokine receptor type 3; C-C CKR-3; CC-CKR-3; CCR-3; CKR3; Macrophage inflammatory protein 1-alpha receptor-like 2; MIP-1 alpha RL2; CD antigen CD193; CCR3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CCR3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 359

• Applicable Applications for CCR3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CCR3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CCR3 sirna

siRNA to inhibit CCR3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157012009
• NCBI GeneID: 12771
• NCBI Accession #: NP_034044.3
• NCBI GenBank Nucleotide #: NM_009914.4
• UniProt Accession #: P51678; Q8K3M7
• Molecular Weight: 41,783 Da
• NCBI Official Full Name: probable C-C chemokine receptor type 3
• NCBI Official Synonym Full Names: chemokine (C-C motif) receptor 3
• NCBI Official Symbol: Ccr3
• NCBI Official Synonym Symbols: CKR3; Cmkbr3; CC-CKR3; Cmkbr1l2
• NCBI Protein Information: probable C-C chemokine receptor type 3
• UniProt Protein Name: Probable C-C chemokine receptor type 3
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: Ccr3
• UniProt Synonym Gene Names: Cmkbr1l2; Cmkbr3; C-C CKR-3; CC-CKR-3; CCR-3; CCR3; CKR3; MIP-1 alpha RL2
• UniProt Entry Name: CCR3_MOUSE

Uniprot Description

CCR3: Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Receptor, cytokine; Membrane protein, multi-pass; Membrane protein, integral; Motility/polarity/chemotaxis; GPCR, family 1

Cellular Component: extracellular space; membrane; plasma membrane; integral to membrane; endosome

Molecular Function: G-protein coupled receptor activity; protein binding; signal transducer activity; C-C chemokine receptor activity; chemokine receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; mast cell chemotaxis; eosinophil chemotaxis; positive regulation of endothelial cell proliferation; chemotaxis; signal transduction; inflammatory response