BMP4 sirna

BMP4 siRNA (Mouse)

Cat. Num. AAA8203192

• Gene Names: Bmp4; Bmp-4; Bmp2b; Bmp2b1; Bmp2b-1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BMP4; BMP4 siRNA (Mouse); BMP-4; DVR-4; Bone morphogenetic protein 4; Bone morphogenetic protein 2B; BMP-2B; BMP4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BMP4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 408

• Applicable Applications for BMP4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BMP4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BMP4 sirna

siRNA to inhibit BMP4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 121949823
• NCBI GeneID: 12159
• NCBI Accession #: NP_031580.2
• NCBI GenBank Nucleotide #: NM_007554.2
• UniProt Accession #: P21275
• Molecular Weight: 46,497 Da
• NCBI Official Full Name: bone morphogenetic protein 4
• NCBI Official Synonym Full Names: bone morphogenetic protein 4
• NCBI Official Symbol: Bmp4
• NCBI Official Synonym Symbols: Bmp-4; Bmp2b; Bmp2b1; Bmp2b-1
• NCBI Protein Information: bone morphogenetic protein 4
• UniProt Protein Name: Bone morphogenetic protein 4
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: Bmp4
• UniProt Synonym Gene Names: Bmp-4; Dvr-4; BMP-4; BMP-2B
• UniProt Entry Name: BMP4_MOUSE

Uniprot Description

BMP4: Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Homodimer; disulfide-linked. Interacts with GREM2. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition. Interacts with SOSTDC1. Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: proteinaceous extracellular matrix; extracellular space; cytoplasm; extracellular region

Molecular Function: heparin binding; protein binding; protein homodimerization activity; growth factor activity; cytokine activity; transforming growth factor beta receptor binding; chemoattractant activity

Biological Process: negative regulation of MAP kinase activity; activation of MAPKK activity; positive regulation of apoptosis; embryonic skeletal development; positive regulation of transcription, DNA-dependent; negative regulation of chondrocyte differentiation; mesodermal cell differentiation; telencephalon regionalization; cardiac muscle cell differentiation; germ cell development; BMP signaling pathway; regulation of protein import into nucleus; anatomical structure formation; kidney development; regulation of odontogenesis of dentine-containing teeth; embryonic limb morphogenesis; endochondral ossification; negative regulation of immature T cell proliferation in the thymus; positive regulation of cardiac muscle fiber development; cell fate commitment; camera-type eye development; regulation of smooth muscle cell proliferation; neuron fate commitment; camera-type eye morphogenesis; regulation of gene expression; negative regulation of mitosis; positive regulation of epidermal cell differentiation; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of apoptosis; tissue development; positive regulation of protein binding; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; inner ear receptor cell differentiation; ureteric bud development; intermediate mesodermal cell differentiation; forebrain development; positive regulation of cell proliferation; embryonic morphogenesis; angiogenesis; positive regulation of BMP signaling pathway; common-partner SMAD protein phosphorylation; negative regulation of T cell differentiation in the thymus; positive regulation of bone mineralization; positive regulation of ossification; odontogenesis of dentine-containing teeth; embryonic skeletal morphogenesis; osteoblast differentiation; positive regulation of osteoblast differentiation; blood vessel endothelial cell proliferation during sprouting angiogenesis; telencephalon development; ureteric bud branching; regulation of cell fate commitment; positive regulation of neuron differentiation; anterior/posterior axis specification; lung development; renal system process; macrophage differentiation; heart development; multicellular organismal development; lymphoid progenitor cell differentiation; post-embryonic development; positive regulation of endothelial cell differentiation; positive chemotaxis; induction of an organ; erythrocyte differentiation; chondrocyte differentiation; specification of organ position; regulation of endothelial cell proliferation; monocyte differentiation; embryonic cranial skeleton morphogenesis; negative regulation of striated muscle development; branching morphogenesis of a tube; mesoderm formation; negative regulation of phosphorylation; positive regulation of endothelial cell proliferation; hemopoietic progenitor cell differentiation; steroid hormone mediated signaling; negative regulation of transcription, DNA-dependent; positive regulation of cell differentiation; metanephros development; alveolus development; positive regulation of epithelial cell proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; embryonic hindlimb morphogenesis; positive regulation of vascular endothelial growth factor receptor signaling pathway; negative regulation of cell cycle; odontogenesis; smooth muscle development; vasculature development; ovarian follicle development; regulation of cell differentiation; regulation of smooth muscle cell differentiation; cell differentiation; skeletal development; dorsoventral neural tube patterning; negative regulation of epithelial cell proliferation; blood vessel development; ossification; negative regulation of oligodendrocyte differentiation; eye development; pituitary gland development; cartilage development; neural tube closure; positive regulation of protein amino acid phosphorylation; negative regulation of myoblast differentiation; mesodermal cell fate determination; growth