BIRC6 sirna

BIRC6 siRNA (Mouse)

Cat. Num. AAA8240187

• Gene Names: Birc6; Bruce; AA501170; mKIAA1289; A430032G04Rik; A430040A19Rik; D630005A10Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BIRC6; BIRC6 siRNA (Mouse); KIAA1289; Baculoviral IAP repeat-containing protein 6; BIR repeat-containing ubiquitin-conjugating enzyme; BRUCE; Ubiquitin-conjugating BIR domain enzyme apollon; APOLLON; BIRC6 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BIRC6 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 4873

• Applicable Applications for BIRC6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BIRC6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BIRC6 sirna

siRNA to inhibit BIRC6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 409971427
• NCBI GeneID: 12211
• NCBI Accession #: NP_031592.3
• NCBI GenBank Nucleotide #: NM_007566.3
• UniProt Accession #: O88738; Q69ZM5; Q8BNX0; Q8BR72; Q8BRV7; Q8C737; E9PYU6
• Molecular Weight: 530,518 Da
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 6
• NCBI Official Synonym Full Names: baculoviral IAP repeat-containing 6
• NCBI Official Symbol: Birc6
• NCBI Official Synonym Symbols: Bruce; AA501170; mKIAA1289; A430032G04Rik; A430040A19Rik; D630005A10Rik
• NCBI Protein Information: baculoviral IAP repeat-containing protein 6
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 6
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: Birc6
• UniProt Synonym Gene Names: Kiaa1289; BRUCE; APOLLON
• UniProt Entry Name: BIRC6_MOUSE

Uniprot Description

BIRC6: Anti-apoptotic protein which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase. Has an unusual ubiquitin conjugation system in that it could combine in a single polypeptide, ubiquitin conjugating (E2) with ubiquitin ligase (E3) activity, forming a chimeric E2/E3 ubiquitin ligase. Its tragets include CASP9 and DIABLO/SMAC. Acts as an inhibitor of CASP3, CASP7 and CASP9. Important regulator for the final stages of cytokinesis. Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification. Homodimer. Binds the activated, processed forms of CASP3, CASP6, CASP7 and CASP9. Interacts with RNF41, DIABLO/SMAC, HTRA2, KIF23/MKLP1, USP8/UBPY, BIRC5/survivin, MAP2K1/MEK1, RAB8A/RAB8, RAB11A/RAB11, PLK1, EXOC3/SEC6 and EXOC4/SEC8. Expressed in brain cancer cells. Inhibited by DIABLO/SMAC, HTRA2, CASP3, CASP6, CASP7 and CASP9.

Protein type: EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19

Cellular Component: Golgi apparatus; spindle pole; cytoskeleton; membrane; cytoplasm; microtubule organizing center; trans-Golgi network; midbody; endosome

Molecular Function: protein binding; protease inhibitor activity; coenzyme F420-2 alpha-glutamyl ligase activity; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ribosomal S6-glutamic acid ligase activity; ubiquitin-protein ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; cysteine protease inhibitor activity; ligase activity

Biological Process: mitosis; cell division; apoptosis; positive regulation of cell proliferation; negative regulation of peptidase activity; protein ubiquitination; regulation of signal transduction; cell cycle; regulation of cytokinesis; negative regulation of apoptosis; placenta development; regulation of cell proliferation