PAX8 recombinant protein

PAX8 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121701

• Purity: >90% as determined by SDS-PAGE
• Synonyms: PAX8; PAX8 Protein; Human; Recombinant (His Tag); Human PAX8 Protein (His Tag); paired box 8; PAX8 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 10% Glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Pro2-Ser146

• Species: Human
• Predicted N Terminal: Pro 2
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mature form of human PAX8 (Q06710-1)(Pro2-Ser146) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PAX8 recombinant protein

Background: PAX8 gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. PAX8 is involved in thyroid follicular cell development and expression of thyroid-specific genes. Also functions in very early stages of kidney organogenesis. Mutations in PAX8 gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

References

Poleev A. et al., 1995, Eur J Biochem. 228 (3): 899-911 Poleev A. et al., 1993, Development. 116 (3): 611-23. Di Palma. et al., 2003, J Biol Chem. 278 (5): 3395-402.

NCBI and Uniprot Product Information

• NCBI GI #: 215273928
• NCBI GeneID: 7849
• UniProt Accession #: Q06710; Q09155; Q16337; Q16338; Q16339; Q4ZG35; Q96J49
• Molecular Weight: 295
• NCBI Official Full Name: Paired box protein Pax-8
• NCBI Official Synonym Full Names: paired box 8
• NCBI Official Symbol: PAX8
• NCBI Protein Information: paired box protein Pax-8; paired domain gene 8
• UniProt Protein Name: Paired box protein Pax-8
• Protein Family: Paired box protein
• UniProt Gene Name: PAX8
• UniProt Entry Name: PAX8_HUMAN

NCBI Description

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]

Uniprot Description

PAX8: Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells. Interacts with WWTR1. Expressed in the excretory system, thyroid gland and Wilms tumors. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; transcription factor activity; thyroid-stimulating hormone receptor activity

Biological Process: regulation of apoptosis; anatomical structure morphogenesis; inner ear morphogenesis; central nervous system development; transcription, DNA-dependent; ureteric bud branching; positive regulation of transcription, DNA-dependent; thyroid gland development; positive regulation of transcription from RNA polymerase II promoter; mesonephros development; kidney development; pronephros development; urogenital system development

Disease: Hypothyroidism, Congenital, Nongoitrous, 2