BEST1 sirna

BEST1 siRNA (Mouse)

Cat. Num. AAA8222781

• Gene Names: Best1; Bmd; Vmd2; mBest1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BEST1; BEST1 siRNA (Mouse); BMD1; VMD2; Bestrophin-1; Vitelliform macular dystrophy protein 2 homolog; BEST1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BEST1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 551

• Applicable Applications for BEST1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BEST1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BEST1 sirna

siRNA to inhibit BEST1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 160333218
• NCBI GeneID: 24115
• NCBI Accession #: NP_036043.2
• NCBI GenBank Nucleotide #: NM_011913.2
• UniProt Accession #: O88870; Q6H1V0; B2RSY6
• Molecular Weight: 63,684 Da
• NCBI Official Full Name: bestrophin-1
• NCBI Official Synonym Full Names: bestrophin 1
• NCBI Official Symbol: Best1
• NCBI Official Synonym Symbols: Bmd; Vmd2; mBest1
• NCBI Protein Information: bestrophin-1
• UniProt Protein Name: Bestrophin-1
• Protein Family: Bestrophin
• UniProt Gene Name: Best1
• UniProt Synonym Gene Names: Bmd1; Vmd2
• UniProt Entry Name: BEST1_MOUSE

Uniprot Description

BEST1: Forms calcium-sensitive chloride channels. Highly permeable to bicarbonate. Defects in BEST1 are the cause of vitelliform macular dystrophy type 2 (VMD2); also known as Best macular dystrophy (BMD). VMD2 is an autosomal dominant form of macular degeneration that usually begins in childhood or adolescence. VMD2 is characterized by typical 'egg-yolk' macular lesions due to abnormal accumulation of lipofuscin within and beneath the retinal pigment epithelium cells. Progression of the disease leads to destruction of the retinal pigment epithelium and vision loss. Defects in BEST1 are the cause of retinitis pigmentosa type 50 (RP50). A retinal dystrophy belonging to the group of pigmentary retinopathies. RP is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Defects in BEST1 are a cause of adult-onset vitelliform macular dystrophy (AVMD). AVMD is a rare autosomal dominant disorder with incomplete penetrance and highly variable expression. Patients usually become symptomatic in the fourth or fifth decade of life with a protracted disease of decreased visual acuity. Defects in BEST1 are the cause of bestrophinopathy autosomal recessive (ARB). A retinopathy characterized by central visual loss, an absent electro-oculogram light rise, and a reduced electroretinogram. Defects in BEST1 are the cause of vitreoretinochoroidopathy autosomal dominant (ADVIRC). A disorder characterized by vitreoretinochoroidal dystrophy. The clinical presentation is variable and may be associated with cataract, nanophthalmos, microcornea, shallow anterior chamber, and glaucoma. Belongs to the bestrophin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, chloride; Membrane protein, integral; Transporter, ion channel; Transporter; Membrane protein, multi-pass

Cellular Component: membrane; basolateral plasma membrane; plasma membrane; integral to membrane

Molecular Function: chloride channel activity

Biological Process: transport; transepithelial chloride transport; detection of light stimulus involved in visual perception; chloride transport; ion transport; regulation of calcium ion transport